الفهرس 
Pituitary GlandOnly 14 pages are availabe for public view
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Abstract
The present research aimed to study the possible effect of chronic
administration of phenobarbital sodium on different endocrine glands in
adult male albino rats using histological, morphometric and hormonal assay
study.
Material and methods: In this experiment 50 adult male albino rats
weighing 150-200 grams each were used. The rats were divided into two
groups: group I (control group): This group contains 10 rats received 0.2 ml
saline/ 100gm body weight. Each rat received about 0.4 ml saline/day orally
by oro-gastric tube for 45 days. group II: Experimental group: this group
contains 40 rats which had been sub-divided into 2 subgroups: group IIa
and group IIb (20 rats each): group IIa: rats of this group received about 15
mg of phenobarbital sodium once daily orally via oro-gastric tube for 30
days. The rats have been sacrificed after 30 days. group IIb: all rats of this
group received 15mg of phenobarbital sodium once daily for 30 days orally
via oro-gastric tube and left without treatment for another 15 days. The rats
have been sacrificed after 45 days. At the appropriate time, the rats of all
groups were anesthetized, sacrificed and subjected to histological,
morphometric and hormonal assay study.
Results: The present study revealed that the chronic administration of
phenobarbital sodium caused degenerative changes in pituitary gland,
thyroid and adrenal glands. The light microscopic examination of the
pituitary gland sections of drug treated rats showed dilatation and
congestion of blood sinusoids between the secretory granules and presence
of many vacuoles in the cytoplasm of secretory granules. The electron
microscopic examination of pituitary gland sections of drug treated rats
showed marked decrease in secretory granules amount and irregularity of
nucleus. There was marked improvement in the light and electron
microscopic examination of pituitary gland section of withdrawal group.