الفهرس 
ContentsOnly 14 pages are availabe for public view
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Abstract
Three phenotypic methods were used for identification against molecular PCR method. Germ tube test which is used for C. albicans identification, 92.3% of C. albicans were germ tube positive as identified truly, and 56.5% C. tropicalis gave false positive germ tube results. For identification by RapIDTM Yeast Plus only one isolate out of 20 was misidentified (5%) and, 19 isolates were correctly identified with RapIDTM Yeast Plus (95%).Identification with Brilliance™ Candida Agar identify 148 isolates out of 164 completely correct (90.2%), while 16 isolates couldn’t be confirmed accurately. Finally using PCR method gave 97.5% of the isolates were accurately identified, while only 4 isolates not identified by PCR (2.4%). The prevalence of C. tropicalis (59.1%) was significantly higher than of C. albicans (31.09%) (P= 0.005).Virulence factors expressed by different Candida species including Phospholipase activity was demonstrated in 52 (31.7%) isolates of C. albicans. 51(31.09%) isolates of C. albicans, and only one (0.6%) isolate C. tropicalis. Secretory aspartic proteinase activity was observed among 101 isolates (61.58%) of Candida isolates. Regarding genetic detection of aspartyl protinase activity, SAP9 gene was detected in 88 (87.12%) while SAP10 gene was detected in 21 (23.8%) isolates. Expression of plasma coagulase enzyme was detected in 132 (80.4%) isolates. Biofilm formation was detected in 114 isolates were biofilm producer.The activity of clove essential oil (EO) was evaluated against 37 isolates of Candida species, then during biofilm evaluation, it was observed that almost no effect on the biofilm activity of all Candida species even with different concentrations or with different contact time.After using EO, isolates of C. tropicalis showed previously (2+) produced after treatment (1+) at 100% concentrations and different contact time also. Isolates of C. albicans isolates showed previously (2+), the protease enzyme activity diminished at 100%concentration.For phospholipase evaluation, the enzyme production inhibited in [4 isolates when treated with 25% concentration of EO (1 isolate after 3hrs, 2 isolates after 8hrs and 1 isolate after 24hrs), 4 isolates when treated with 50% concentration (2 isolates after 8hrs and 2 isolates after 24hrs), and 1 isolate when treated with 100% concentration after 8hrs].