الفهرس 
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Abstract
The present study aims at evaluating the effect of tomato juice alone and combined with broccoli on experimentally induced: Non-alcoholic fatty liver induced by high fat and high fructose diet in rats. Liver fibrosis induced by CCL4 in rats. Four groups of rats were used according to the model: group 1: received standard chow pellets and tap water. group 2: received HF + HFr or CCL4 (according to the model) in addition to chow pellets and tap water.group 3: received tomato juice added to drinking water (50% w/v) in addition to HF+ HFr or CCL4. group 4: received tomato juice added to drinking water (50% w/v) and broccoli florets and stems (10% w/w) added to diet in addition to HF + HFr or CCL4 Feeding HF + HFr diet daily for 16 weeks induced non-alcoholic fatty liver and administration of CCL4 2 times weekly for 8 weeks induced liver fibrosis. Both were indicated by high levels of liver function biomarkers including ALT, AST, total bilirubin and GGT and reduced albumin level. Also, HF + HFr diet and CCL4 resulted in increase in lipid profile parameters including cholesterol, triglycerides and LDL-cholesterol and decrease in HDL-cholesterol. Additionally, HF + HFr diet and CCL4 resulted in generation of oxidative stress indicated by increase in hepatic tissue MDA level and decreased tissue GSH and SOD levels. Considering mRNA expression of HMG-CoA reductase and CYP2E1, they were elevated upon chronic HF + HFr feeding. While there was a decrease in PPARγ expression level. Considering TGF-β1 and PDGF-B, CCL4 caused significant increase in both of them. These changes were confirmed by histopathological evaluation using hematoxylin & eosin and masson trichrome stains. Consumption of tomato juice either alone or combined with broccoli concomitantly with HF + HFr diet succeeded to attenuate HF + HFr diet induced non- alcoholic fatty liver. This was indicated by decreasing hepatic inflammation as revealed by examined liver sections histopathologically. The improvement was ensured by improving liver function parameters, nearly normalization of lipid profile biomarkers and reducing lipid peroxidation (low MDA levels) through restoring the antioxidant GSH and SOD hepatic capacity with a decrease in NO hepatic level. Combining tomato juice with broccoli chowed better effects on GSH and SOD and only the combination could significantly reduce MDA level. Expressions of HMG-CoA reductase, CYP2E1 were lowered as well. Both tomato juice and combined tomato juice with broccoli activated PPARγ and significantly increased its level. It could be concluded that tomato and broccoli supplementation may alleviate the harmfull effects resulted from experimentally induced non-alcoholic fatty liver and also may have a role in attenuating experimentally induced liver fibrosis. Further studies are required for these results to be confirmed.