الفهرس 
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Abstract
Summary
This study aimed to assess the prevalence of mecC gene among 600 methicillin resistant staphylococcal isolates (520 methicillin resistant S. aureus and 80 methicillin resistant coagulase negative staphylococci) collected from two university hospitals in Egypt.
Isolates were tested as follows:
All 600 isolates were screened by disc diffusion for cefoxitin (30ug) and oxacillin (1ug) to detect isolates that show oxacillin susceptibility while cefoxitin resistant, as a method to predict the potential existence of mecC gene in those isolates of discordant susceptibility.
Discordant isolates included in selected 150 isolates (110 MRSA and 40 MR-CoNS) were tested by PBP2a latex agglutination test, and or conventional PCR for mecA gene.
Samples negative for mecA gene were tested by Vitek 2 for MIC of cefoxitin and oxacillin and for mecC gene detection by PCR.
The results were as follows:
Discordant susceptibility was displayed in 4 (2%) MRSA isolates and 5 (6%) MR-CoNS isolates.
These discordant isolates were tested positive by both PBP2a latex agglutination test and PCR for mecA gene. However the 6 (5.5%) negative isolates for mecA were otherwise resistant to both cefoxitin and oxacillin by disc diffusion method.
The 6 mecA negative isolates were both cefoxitin and oxacillin resistant (MIC ≥4 ug/ml) by Vitek 2.
Those mecA negative isolates were also negative for mecC gene by PCR.
Conclusion:
The gene for methicillin resistance mecC couldn’t be detected in this study with zero prevalence which may be a base line for further prevalence studies that may detect future change in prevalence of that gene.