الفهرس 
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Abstract
Background and objectives: The imperative role of dental pulp stem cells (DPSCs) in regenerative therapy demands an in-vitro expansion which must deal with the safety and ethical problems associated with fetal bovine serum (FBS). The primary aim of this study was to compare the effects of human platelet rich fibrin (hPRF) exudates Vs FBS on proliferation and osteodifferentiation of human dental pulp stem cells (hDPSCs). The secondary one was to determine the optimum concentration of hPRF exudates inducing hDPSCs proliferation and osteodifferentiation. Materials & methods: The direct method was used to prepare hPRF exudates. Twelve impacted third molars were used for isolation of hDPSCs by the out growth expansion technique. For cell viability and proliferation rate testing, 96 well plates were used and the assay was done in duplicate and the trail repeated four times under the same conditions. Six wells were used to contain 10% FBS, serum free media, 1%, 5%, 10% and 20% concentrations of hPRF exudates, respectively. The proliferation assay was carried out by MTS tetrazolium cell proliferation assay kit and Elisa reader. The study design for osteodifferentiation protocol was exactly as the proliferation one and instead the assay was carried out by alizarin red with Elisa reader. Results: Compared to 10%FBS, 10%hPRF exudates was the optimum concentration for hDPSCs proliferation, while 1%hPRF exudates were the optimum concentration for osteodifferentiation of hDPSCs. Conclusion: Avoiding the risk of zoonoses which may be occurred with FBS, it is recommended to use 10%hPRF exudates for proliferation and 1% for osteodifferentiation.