الفهرس 
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Abstract
Eugenol, the main phenolic component of EOs of some aromatic plants, was evaluated for its in vitro and in vivo anticandidal activity. Therapeutic efficacy in the treatment of experimental oral candidiasis induced by C.albicans in immunosuppressed rats was assessed. This anticandidal activity was analyzed by microbiological and histopathologi¬cal techniques, and it was compared with that of fluconazole, which was used as a positive control.
Five C. albicans strains were used for antifungal assay, including two strain of human origin from oral and vaginal candidiasis, one strain from cattle rectum (diarrhea), other strain from turkey suffering from crop mycoses and one reference strain ATCC 90028 obtained from the department of Microbiology, Faculty of Veterinary Medicine, Zagazig University.
Antifungal activity of eugenol and fluconazole was assessed using disc diffusion and broth micro dilution method. Inhibition zones of eugenol ranged from 5-36 at concentration of 100% mm in diameter in comparison to fluconazole which ranged from 17 to 50mm. MIC ranged from 1-16µg/ml and 8 to 32µg/ml for eugenol and fluconazole, respectively. The concentrations used for the treatment of rats were 2×MIC for eugenol and fluconazole. This treatment began 72 h after the inoculation and continued for 8 days thereafter at a dose of 32µg/ml and 16µg/ml for eugenol and fluconazole, respectively by topical application on oral cavity.
Efficacy evaluation of eugenol in the treatment of experimental oral candidiasis was performed in immunosup¬pressed rats. A total of twenty apparently healthy male white albino rats with average body weight of.