الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
•The present study was carried out on 837 animals ( 626 sheep and 211 goats) from different localities of Sharkia governorate to study the prevalence of brucellosis, diagnosis of brucellosis by using different serological techniques including (BAPAT, RBPT and CFT) and evaluate these techniques, Apply trials of isolation, identification and typing of Brucella spp. from seropositive animals and characterize the Brucella spp. which isolated from seropositive animals using polymerase chain reaction (PCR) .
• All blood samples are tested by BAPAT and RBPT as screening tests and confirmed by CFT.
• Serological examination of 837 blood samples under investigation by the application of :-
A- BAPAT: revealed that total percentage of positive reactors was 14/626 total sheep samples (2.2 %) and 11/211 total goat samples (5.2%).
B- RBPT: revealed that total percentage of positive reactors was 10/626 total sheep samples (1.6%) in sheep and 10/211 total goat serum samples (4.7%).
C- CFT: revealed that total percentage of positive reactors was 12/626 total sheep samples (1.9%) in sheep and 9/211 total goat serum samples (4.3%).
• Sensitivity and specificity of BAPAT with CFT was 100% and 99.5% respectively, While RBPT was 90.5% and 99.8% respectively.
• The agreement among BAPAT and RBPT by using CFT as gold standard test on 626 and 211 sheep and goats sera by using Cohen’s Kappa statistics showed that there were a very good correlation between negative and positive results of (BAPAT and CFT) and (RBPT and CFT) where the agreement was (0.93) among sheep. And there were a very good correlation between negative and positive results of BAPAT and CFT where the agreement was (0.89) and RBPT and CFT where the agreement was (0.94) among goats.