الفهرس 
Literature reviewOnly 14 pages are availabe for public view
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Abstract
It explained the nature of quinolones and fluoroquinolones, pharmacokinetics, clinical significance, mechanism of action and their classification.
Part ӀӀ: Literature review
This part included a review of the physical and chemical properties of the studied quinolones contining drugs, Ofloxacin (OFX), Moxifloxacin (MFX), Ciprofloxacin (CFX), Lomefloxacin (LMFX) and Danofloxacin (DFX). Also, it included different analytical methods reported in the literature that aimed for determination of the chosen drugs in pure form, pharmaceutical formulations and biological fluids.
Part ӀӀӀ: chromatographic determination of Ofloxacin
Two high performance liquid chromatographic method were developed for determination of Ofloxacin. The first method is used for determination of a mixture of Ofloxacin, Dexamethasone and Benzalkonium chloride, while the second method is used for determination of a mixture of Ofloxacin, Cefixime and Ofloxacin Related compound A.
This part involved two sections:
Section A: Isocratic chromatographic determination of Ofloxacin and
Dexamethasone in presence of in active Benzalkonium chloride as a
Preservative.
This section is concerned with the application of HPLC method for the determination of a mixture of Ofloxacin, Dexamethasone and Benzalkonium chloride. The mobile phase of choice was acetonitrile:0.1%perchloric acid: methanol in ratio (65:30:5, v/v), and UV detector was set at 294,240,214 nm. for Ofloxacin, Dexamethasone and Benzalkonium chloride, respectively. The proposed HPLC method is simple, accurate, precise, and can be successfully applied for routine analysis in quality control laboratories for the quantitative determination of Ofloxacin, Dexamethasone and Benzalkonium chloride in pure form and in combined pharmaceutical formulations.
Section B: Isocratic chromatographic estimation of Ofloxacin and Cefixime in presence of the inactive Ofloxacin USP Related compound A
This section is concerned with the application of HPLC method for the determination mixture of Ofloxacin, Cefixime and Ofloxacin USP Related compound A.
The mobile phase of choice was 0.1M ammonium acetate buffer: acetonitrile (35:65) v/v and the pH was adjusted at 6.0 by addition of glacial acetic acid. UV detector was set at 294nm.
The proposed HPLC method is simple, accurate, precise, and can be successfully applied for routine analysis in quality control laboratories for the quantitative determination of Ofloxacin, Cefixime and Ofloxacin USP Related compound A in pure form and in combined pharmaceutical formulations.
Part ӀV: Spectrophotometric determination of some quinolone containing
drugs
This part involved the development of different modes of UV- spectrophotometry for analysis of the studied drugs; Moxifloxacin HCl and Ciprofloxacin HCl with further application on laboratory prepared mixtures and pharmaceutical formulations.
This part involved two sections:
Section A: Spectrophotometric determination of Moxifloxacin HCl and Dexamethasone Sodium Phosphate in presence of Benzalkonium chloride
The zero-order absorption spectra (D0) of Moxifloxacin, Dexamethasone sodium phosphate and Benzalkonium chloride show overlapping, which allows the analysis of Moxifloxacin HCl in presence of Dexamethasone sodium phosphate and Benzalkonium chloride at λmax= 294 nm, but hinders the analysis of Dexamethasone sodium phosphate in presence of Moxifloxacin HCl and Benzalkonium chloride. So, different spectral manipulating techniques (1DD, Aiso, RS, RDSM and MCN ) have been applied to determine Dexamethasone sodium phosphate without interference from Moxifloxacin or Benzalkonium chloride.
Section B: Spectrophotometric determination of Ciprofloxacin HCl and Metronidazol
The zero-order absorption spectra (D0) of Ciprofloxacin HCl and Metronidazol show overlapping, which hinders the analysis of Ciprofloxacin HCl and Metronidazol. Two spectral manipulating techniques (RDSM and DWL) have been applied to determine Ciprofloxacin HCl and Metronidazol.
Part V: Potentiometric determination of some quinolone containing drugs
This part included the determination of Lomefloxacin HCl and Danofloxacin mesylate using ion selective electrodes. This part involved three sections.
Section A: Introduction to ion-selective electrodes (ISEs).
It described the different types of ion selective electrodes and their assembly
Section B: Stability indicating ion selective electrodes for determination of Lomefloxacin HCl (LMFX) in presence of its acid degradation product
This section describes the use of tetraphenyl borate (TPB) as precipitating agent and 2-hydroxy propyl β-cyclodextrin as ionophores on the fabrication of novel sensors for determination of LMFX in presence of its acid degradation product. Sensor 1and 2 were fabricated with dioctyl phthalate as a plasticizer in apolymeric matrix of polyvinyl chloride. While sensor 3 is a carbon paste electrode, these sensors were used for determination of LMFX in its pure form, pharmaceutical formulations and biological fluids. The performance of the proposed sensors were assessed according to the IUPAC recommendations and showed that the sensors had fast, stable and nernestian response.
Response time, effect of pH and temperature on response, selectivity and stability of the electrodes were studied to determine the optimum conditions for the determination of LMFX using the proposed sensors.
Section C: Potentiometric determination of Danofloxacin mesylate (DFX) by using ion selective electrodes
This section describes the fabrication of three novel sensors for determination of DFX in its pure form, pharmaceutical formulations and biological fluids. The performance of the proposed sensors were assessed according to the IUPAC recommendations and showed that the sensors had fast, stable and nernestian response.
Response time, effect of pH and temperature on response, selectivity and stability of the electrodes were studied to determine the optimum conditions for the determination of DFX using the proposed sensors.
Part VӀ: General discussion
General discussion about the different analytical methods which develop in this thesis for determination of certain Fluroquinolones drugs; namely Ofloxacin, Moxifloxacin HCl, Ciprofloxacin HCl, Lomefloxacin HCl, Danofloxacin mesylate in bulk, mixtures, pharmaceutical formulations and biological fluids.
References and Arabic summary
This thesis contains 267 references, 60 tables and 48figures and ended with a summery in Arabic.