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Abstract The present study was aimed to determining the phenotypic and genotypic characteristics of A. hydrophila complex isolated from fresh and frozen beef and poultry meat and determining the pathogenecity of some isolates and determining the source of contamination of meat which constituting a potential zoonotic potentials. To fulfil these objectives, a total number of 214 swab samples were collected from 66 fresh beef, 43 fresh poultry meat, 57 frozen beef, and 34 frozen poultry meat. In addition, nine swabs from hands workers who were handling meat as well as five water samples used for washing in the slaughterhouse and retail markets selling meat. Fresh samples were collected from the slaughterhouse and public live bird markets before chilling of the carcasses or meat processing. While frozen samples were collected from retail markets in Ismailia city, Egypt during the period from March to October 2012. Fresh samples were collected from the slaughterhouse were collected from the neck and the flank region of each whole cattle beef and the neck and the around visceral cavity of each eviscerated poultry carcass. These regions are the most likely to exposure to contamination, either the animal itself or the surrounding environment such as workers. In addition, nine swabs of worker hands were collected from different places of work as following four samples of abattoir, three samples of poultry slaughterhouse and two samples of local retail shops. Samples were examined by both standard microbiological biochemical examination followed by molecular examination testing. Some of virulence factors genes were examined by PCR techniques including aerolysin, lipase, and enterotoxin genes. Results revealed that growth of Aeromonas spp. on different media containing Ampicillin indicated the highly resistance of Aeromonas spp. to Ampicillin. The phenotypic study of isolated strains from different source of origin (beef, poultry and worker hands) revealed that all isolated were typical in their morphological, cultural and biochemical reactions characters. |