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Abstract The following findings were obtained: Chemical analysis of water and mud as well as newly reclaimed soil samples around the biggest eight hypersaline, alkaline lakes of Wadi-El-Natrun The chemical analysis revealed that there are significant variations between water, mud and soil samples of Wadi-El-Natrun lakes in their chemical composition. Lower amounts of TDS, Na+, K+, CO3 -2, HCO3 - , Ca+2, Mg+2, Cl- registered in soil than those recorded in water and mud may explain the highest number of propagules in soil than those in water and mud, since stresses from pH, TDS, Na+ and other cations and anions are much lower. Mycological analysis 1- Mesophilic, halophilic/halotolerant and thermophilic/thermotolerant fungi were isolated from the four habitats (air, water, mud and newly reclaimed soil) of the biggest eight hypersaline, alkaline lakes of Wadi-El-Natrun in addition to the osmophilic fungi from the air. 2- Morphological characteristics were used to identify the isolated fungi and in many cases the internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA were used to confirm the identification. 3- A total of 157 species related to 52 genera represented by 955 isolates (885 identified and 70 unidentified) isolates of mesophilic, halophilic/halotolerant and thermophilic/thermotolerant fungi were isolated from air, water, mud and newly-reclaimed soil samples using four isolation media and two incubation temperatures. |