الفهرس 
Cover Page Title in EnglishOnly 14 pages are availabe for public view
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Abstract
Helicobacter pylori considered the most important pathogens as a cause of gastric and duodenal ulcer as well as gastric cancer in human and pets. Also E.coli is considered a major cause of enteritis in human and pets. This work was carried out to investigate the presence of H.pylori and E.coli in stool of human and pet animals through: 1-Isolation and serodiagnosis of H.pylori in stool of human and pet animals 2-Isolation and serotyping of E.coli 3- characterization of some virulence factors present in E.coli isolates Our study revealed that H. pylori was detected in stool of humans and cats at a percentage of 52.8 % and 33.3 %, respectively. On the other hand, H. pylori could not be isolated from dogs. Detection of H. pylori antigen from human stool indicated that 45 out of 50 (90%) of examined samples were positive for H.pylori. H. pylori antigen could not be detected among dogs and cats stool. Detection of H. pylori antigen titer from human stool revealed that the positive samples gave titers ranged from (1.88 to .45). E.coli was isolated from cats, dogs and human at percentage of 79.2%, 75% and 77.3%, respectively. Haemolysis produced by E. coli isolated from Human, Dogs and Cats showed that E. coli isolated from Human gave alpha, beta and gamma hemolysis at percentage of 47%, 17.6% and 35.2% respectively while E.coli isolated from Dogs gave alpha, beta and gamma hemolysis at percentage of 56.2%, 12.5% and 31.2%, respectively and E. coli isolated from Cats gave alpha, beta and gamma hemolysis at percentage of 63.1%, 10.5% and 26.3%, respectively. Serotyping of E.coli isolates revealed that serotypes (O146, O20 and O125) were detected in cat, serotypes (O55, O114 and O1) were detected in dog and serotypes (O86, O20 and O1) were detected in human. PCR identification of E.coli isolates gave positive results at 720 bp, Iss gene gave positive results at 266bp, Fim gene gave positive results at 508 bp and Tsh gene was not detected in any of the isolates. Iss and Fim gene were detected in human, dog and cat at percentage of 100%, 100% and 66.7%, respectively.