الفهرس 
ReviewOnly 14 pages are availabe for public view
 |  | from | 153 |  |  |
| | | from | 153 | | |
Abstract
The biochemical effect of curcumin on some osteoarthritic markers in blood of rats injected MIA induced osteoarthritis at a dose of (200 mg/kg b.wt) was investigated. Thirty six, 12-weeks old male, albino rats weighing between 100-150 g, were obtained from the Experimental Animal Centre of Faculty of Veterinary Medicine, Benha University and were used in this study. Design of the experimental work: Rats were divided into four groups, each group contained 9 rats as follow:- Group1 (normal control group):were not subjected to any intervention and were used as controls. group 2 (osteoarthritic group) : received intra-articular injection of 2 mg /kg (b.w.) of MIA (Crystal Powder M=185.96g/mol, Germany, Sigma) and OA was induced by this method only in the right knees of the animals. group 3 (osteoarthritic and treated group with curcumin): received intra-articular injection of 2 mg/kg (b.w.) MIA and were received 200 mg/kg (b.w.) of curcumin orally /day for 28 days. group 4 (curcumin normal group): received 200 mg/kg (b.w.) of curcumin orally /day for 28 days. Within each intervention group, the rats were further grouped into three small subgroups consisting of three animals per subgroup for each of the 2 different analysis (biochemical and molecular analysis). Rats were fasted overnight and subjected to light diethyl ether anaesthesia before sacrifing on day 29th. The articular tissue samples were collected. Articular cartilage samples were used for determination of the following parameters: Reduced Glutathione (GSH) , Superoxide dismutase (SOD) Malondialdehyde (MDA) , Catalase (CAT) Glutathione peroxidase (GPx) Glycosaminoglycan (GAG) Tumer necrosis factor-α (TNF-α) Transforming growth factor-β1 (TGF-β1).