الفهرس 
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Abstract
The water buffalo represents a vital part of the agricultural economy in Egypt and other developing countries. Moreover, Buffaloes have low reproductive efficiency which could be attributed to the low total number of follicles in the ovary, poor superovulatory response and high percentage of atretic follicles. Recently, efforts are established to improve the reproductive efficiency of these animals using reproductive biotechnology as in vitro fertilization (IVF) and embryo transfer (ET).
Some studies have been shown that the in vitro culture medium in which the embryos are cultured after fertilization is the most important determinant of the quality of embryos. The evaluation of embryo quality is one of the most critical factors, which determine the output of embryo transfer. Its significance is attributed to the essential relationship between embryo quality and its developmental stage.
The present study aimed to:
1. Assess the developmental competence of the in vitro produced embryos under different culture conditions.
2. Qualify the in vitro produced embryos under different culture conditions (addition of granulosa and oviductal cell monolayers, epidermal growth factor (EGF), lactoferrin (LF) and vitamin B12 on culture medium) using different qualification methods including:.