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Abstract The present study was conducted for a period of one year extending from October 2015 to September 2016 which investigate level of infection by endo-parasites in Egyptian equines through fecal examination of (260) fecal samples, post mortem examination of remains of (40) donkeys at Giza zoo in addition to application of some molecular biology techniques as a new diagnostic tool. Fecal examination revealed that 58.5% of equines were infected with at least one type of endoparasites. The post mortem examination revealed the presence of Habronema muscae, 2nd and 3rd larval stages of G. intestinalis and G. nasalis, Dictyocaulus arnfieldi and hydatid cysts. Using molecular tools, the DNA extracted from protoscolices collected from hydatid cysts were subjected to Polymerase Chain Reaction (PCR) and sequencing. All the examined samples were showed homology to reference nucleotide sequences of E. equinus accessed in Genbank. Thirty randomly selected fecal samples were subjected to PCR amplification for detection of DNA of Habronema spp. A total of 12 (40%) samples were positive for H. microstoma and 4 (13.3%) samples were positive for H. muscae and there were no cases of mixed infection by both H. muscae and H. microstoma. |