الفهرس 
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Abstract
There are many methods for identification of dermatophytes including phenotypic and genotypic methods. Recently, MALDI-TOF mass spectroscopy is increasingly being used for identification of dermatophytes.
This study aimed to investigate the capability of RT-PCR andMALDI-TOF MS to identify and discriminate between the most frequent dermatophyte species isolated from human and animals in comparison with conventional methods. To that end, dermatophyte isolates were identified by phenotypic method, RT- PCR and MALDI-TOF mass spectroscopy analysis.
A total of 200 skin scraping and hair samples were collected from human and animal dermatophytosis (100 from each), 40 case were positive in case of human (40%) while 61 case in animals (61% represented in 30 isolates from cat, 20 from dog, 5 from cattle and 6 from horse). The most predominant clinical case in human dermatophytosis was tinea capitis (40%) followed by tinea faciei (25%) and tinea corporis (20%). M. canis was the most isolated species from tinea capitis (30%) and tinea faciei (9%) followed by T. violaceum (2% and 1% for tinea capitis and tinea faciei respectively). While tinea corporis mainly caused by M. canis (35%), T. mentagrophytes (2%) and T. violaceum (1%). On the other hand, the predominant species identified in case of cat ringworm was M. canis(86.7%) followed byT. mentagrophytes(6.7%) and T. verrucosum(6.7%). While in dog was M. canis(100%), in cattle was T. verrucosum(100%) and in horse were T. verrucosum(66.7%)followed by T. mentagrophytes(33.3%).