الفهرس 
Introduction and Aim of the studyOnly 14 pages are availabe for public view
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Abstract
Autologous Fat transplantation is frequently applied for aesthetic and
reconstructive breast conditions .However, the most common limitation of
fat transplantation is the long-term unpredictability of volume
maintenance. Regenerative cell based strategies such as the use of PRP
hold tremendous promise for breast augmentation and correction of breast
soft-tissue defects and deformities and offer the possibility of fulfilling the
key principle of replacing like with like as an aesthetic filler, without the
drawbacks of conventional autologous Fat grafting.
It is noted that most of the encountered limitations and complications with
fat grafts procedures related to excessive handling, and major trauma
during the harvesting, processing, and delivery of the autologous grafts to
recipient bed and new environment at the recipient site ,so fat survival is
technique dependent.
Adipose tissue is fragile living tissue. Standardization of techniques is
needed to improve the results and satisfaction with structural fat grafting
in both small and large-volume applications. Gradual standardization of
harvesting, manipulation, delicate handling and transfer protocols is
improving the ability to accurately predict volume enhancements and
appreciate the long-term survival of the grafted tissues as follow:
(I) Preoperative planning ; A thorough patient evaluation and careful
patient selection will lead to improved outcomes; the reciepient site with
good existing subcutaneous tissue, the skin over the breast is thinner and
not tight, a suitable donor site ( lower abdomen and medial thigh ), the
amount of fat graft required.
(II) Fat harvesting; General principles of sterile technique should be
observed at all stages of the procedure. In general, about 1 mL of
tumescent solution is injected for every 1 mL of lipoaspirate to be
extracted. At least 10 minutes are needed for the vasoconstrictive effects
to set in before fat extraction can be performed. The closed-syringe system.