الفهرس 
Introduction and Aim of the Work
Hepatitis C VirusOnly 14 pages are availabe for public view
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Abstract
This study involved fifty six individuals classified into two groups: chronic HCV group included twenty patients and SVR group after triple therapy with sofosbuvir, PEG-INF α and ribavirin included thirty six patients. The patients were recruited from the Unit of Treatment of Viral Hepatitis, Ministry of Health, Assiut district during the period from August 2015 to April 2016.
All patients were subjected to the following:
1- Routine investigations: Complete blood count, AST, ALT, HCV Ab, HBsAg, PCR for HCV RNA andANA.
2- Special investigation:
* Flow cytometric analysis of natural killer cells, their natural cytotoxicity receptors (Nkp30, Nkp44, Nkp46) and their cytoplasmic contents of perforin and granzymeB
* KIR (KIR2DL1, KIR2DL2, KIR2DL3, KIR2DS1, KIR2DS2 and KIR2DS3)and
HLA-C (1 And 2) genotyping by Real time PCR.
The following results were found
1- There was significant decrease in total leucocytic count, absolute lymphocytic count and platelets count in SVR than chronicgroup.
2- There was insignificant increase in NK cells number in SVRgroup.
3- There was a statistical significant decrease in MFI of Nkp46 and statistical significant increase in MFI of granzyme B in SVR than chronicgroup.
4- There was significant positive correlation between Nkp46 and Nkp44 expression and MFI of both perforin and granzyme B in chronic group and significant positive correlation between Nkp46, Nkp44 and Nkp30 expression and MFI of both perforin and granzyme B in SVRgroup
5- The chronic groupshowed
- High frequencies for KIR2DL1 and KIR2DL2 (90%) and low frequency for KIR2DL3 (35%).
- HLA-C1C2 was present in (90%), HLA-C1C1 (5%) and HLA-C2C2(5%)
- Homozygous 2DL1/C1C2 and heterozygous 2DL2/2DS2/C1C2 showed the highest presentation (45%) followed by heterozygous 2DL1/2DS1/C1C2 (40%) while homozygous 2DS1/C1C2 and 2DS1/C2C2 showed the least presentation(5.2%).
6- Regarding SVR group, it showed high frequencies for KIR2DL1 (94.4%), KIR2DL2(86.1%),KIR2DS2(88.9%)andKIR2DS3(86.1%)andshowedlow
frequency for KIR2DL3 (30.6%). And as for HLA-C, HLA-C1C2 was present in (72.2%), HLA-C1C1 (5.6%) and HLA-C2C2 (22.2%). Also heterozygous 2DL1/2DS1/C1C2 showed the highest presentation (63.8%) followed by heterozygous 2DL2/2DS2/C1C2 (38.8%) while homozygous 2DL2/C1C2, 2DL2/C1C1 and heterozygous 2DL2/2DS2/C1C1 showed the least presentation (2.7%).
7- Different associations were found between KIR genotypes (patients carry more inhibitory, more stimulatory or equal number of inhibitory and stimulatory genes) and NK activity measured by (NCRs, perforin, granzyme B and absolute NK count) in bothgroups.