الفهرس 
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Abstract
The present study was carried out to evaluate the hepatoprotective effect of aqueous extracts of dill and rosemary by using carbon tetrachloride as hepatotoxic model and Silymarin as a standard hepatoprotective and their effects were determined using Biochemical tests and histopathological changes. The experimental design was as follow: Thirty rabbit were divided into 6 equal groups.
Preparation of tissue homogenate:- Each sample was homogenized in distilled water using electrical homogenizer, centrifuged at 3000 r.p.m. for 15 minutes. The resulting supernatant were collected and used for estimation of antioxidant enzymes. Tissue homogenates were preserved at -20O C until performing the investigations.
Biochemical determinations:
1-Effect on tissue antioxidant activities: Adminstration of CCL4 (1.5 ml /kg b .wt) I/P (diluted 1:7 in saline ) twice a week for2 successive weeks revealed a marked decrease in tissue activities of SOD, GPX and CAT in comparison with normal control group .
While all groups showed significant increase in tissue SOD and CAT in the order of: [CCL4 +2 extracts] followed by Silymarin then Rosemary and finally dill compared to CCl4-treated rabbits.
Effects on tissue MDA: The I/P injection of CCL4 (1.5 ml/kg b .wt) (diluted 1:7 in saline) twice a week for 2 successive weeks elicited a marked increase in tissue MDA .
B-Biochemical Blood Result:
1-Effects on serum antioxidant parameters: The I/P injection of CCL4 (1.5 ml /kg b .wt) (diluted 1:7 in saline )twice a week for successive 2 weeks elicited a marked decrease in serum activities of SOD, GPX and CAT
Result of serum MDA: The I/P injection of CCL4 (1.5 ml/kg b.wt.) (diluted 1:7 in saline ) twice a week for 2 successive weeks elicited a marked increase in serum MDA .
Effects on liver function parameters: The I/P injection of CCL4 (1.5 ml /kg b.wt) (diluted 1:7 in saline) twice a week for 2 successive weeks elicited a marked elevation in serum activities of ALT, AST and ALP in comparison with normal control group.
Result of total protein and ALB: The I/P injection of CCL4 (1.5 ml/kg b .wt) (diluted 1:7 in saline) twice a week for 2 successive weeks elicited a marked decrease in total protein and ALB.
The histopathological study revealed that The combination of plant extracts showed significant and most effective improvement in reduction in both the inflammatory reactions and degenerative changes, as well as, reduction of fibrosis produced from experimental liver injury using CCl4 induced hepatotoxicity models in rabbits as compared to silymarin alone or each extract alone indicating the potential role of combination treatment in preventing oxidative stress mediated damage and strengthening the mechanism of antioxidant defense.
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