الفهرس | Only 14 pages are availabe for public view |
Abstract Foot and Mouth Disease (FMD) is a highly contagious vesicular virus disease of buffaloes, cattle, sheep, goats, wild ungulates (giraffe and deer), as well as pigs and other clovenhoofed wild and domestic mammals The present study was conducted on forty five native breed Egyptian cattle (female non pregnant) aged 1-5 years. These cattle were belonging to different localities in port- Said Governorate (Bahr El-Baker, El kabboti El Gadid, El Radwan, El Asher and Sahl Tena) in Egypt during FMD outbreak (2016-2017). The animals were divided into two main groups. group (1): 15 apparently healthy cattle considered as control group. This group subdivided into: (gp.1a): animals at age 1- 1.5 year, (gp.1b): 2-3 years and (gp.1c): 4-5 years. group (2): 30 cattle manifested the characteristic clinical signs of FMDV infection. This group subdivided into: (gp.2a): animals at age 1-1.5 year, (gp.2b): 2-3 years and (gp.2c): 4-5 years, 10 animals in each. Two venous blood samples were collected from jugular vein of cattle. The 1st sample (about 2 ml) of blood was taken in sterile vacuum tube (with dipotassium salt of EDTA) used for hematological studies. The 2nd sample (about 10ml) was taken in sterile vacuum plain tube without anticoagulant for serum separation for biochemical, serological and oxidative profiles. Samples for molecular biology study (PCR): Tongue epithelium and vesicular fluid were collected in sterile tube (with glycerol saline) for virus isolation for PCR. |