الفهرس 
Previous work
ACUTE MYELOID LEUKAEMIAOnly 14 pages are availabe for public view
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Abstract
Background: Cancer is an increasingly important cause of morbidity and mortality. Haematological malignancies represent approximately 7% of all malignant diseases. Acute myeloid leukaemia (AML) is an aggressive and fatal disease caused by the replacement of normal marrow elements by leukaemic blasts, and accumulation of immature progenitor cells in the bone marrow. Tremendous efforts have been made to classify AML depending upon cytogenetics and molecular markers. AML treatment basically remained unimproved along the last 20 years; this treatment involves induction of cytotoxic chemotherapy (cytarabine and anthracycline), with minor modifications for elderly patients.
Mesenchymal stem cells (MSCs) derived from umbilical cord (UC) or other tissues are currently being investigated for an ever-expanding number of clinical indications based on their tissue-regenerative, immunomodulatory, and anti-inflammatory effects.
The aim of the present work is to detect the effect of the human mesenchymal stem cells (MSCs) on human Acute Myeloid Leukaemia (AML). Samples of mesenchymal stem cells (MSCs) were separated from human umbilical cord blood (HUCB) and co-cultured with peripheral blood samples of adult acute myeloid leukaemia patients prior to chemotherapy.Methods: The present study included four groups of cultured cells as follows:
group 1: (control group): 6 peripheral blood samples derived from healthy humans.
group 2: (acute myeloid leukaemia blast): 10 peripheral blood samples derived from 10 adult humans diagnosed with acute myeloid leukemia (AML).
group 3: (mesenchymal stem cells, MSCs): 10 human umbilical-cord blood samples.
group 4: (MSCs co-cultured with AML): 10 co-cultured samples.
Flow cytometric analyses were done to identify all the cultured cells (AML and MSCs). Leukaemia inhibitory factor gene (LIF) and the cytokine “interleukin-10” (IL-10) were measured using real-time polymerase chain reaction (qPCR) and ELISA techniques, respectively before and after the co-culture; aiming to evaluate the immunomodulatory and anti-inflammatory effects of MSCs on AML.
Results: In the present study, the group of the acute myeloid leukaemia cells co-cultured with mesenchymal stem cells (MSCs) separated from human umbilical cord blood (HUCB) (group 4) showed a significant increase in the expression level of LIF gene compared with the acute myeloid leukaemia group (group 2). The group of acute myeloid leuakemia cells co-cultured with mesenchymal stem cells (group 4) showed a significant decrease in the IL-10 concentrations compared to that of the acute myeloid leukaemia group (group 2).