الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Polyprolene meshes are widely used for treatment of stress urinary incontinence (SUI) and pelvic organ prolapse (POP). It is safe and effective modality as a mid-urethral sling. However, current mesh materials and POP devices are subject of revisions. FDA and European commission Scientific Committee on Emerging and Newly Identified Health Risks (SCENIHR) elaborated the need for novel regenerative solutions. Alternative biological materials presented questionable durability, doubtful purity and unfavorable host reaction (e.g. decellularized collagen scaffolds). Accordingly, there has been a push for a novel mesh biomaterial to fill the current need.
We aimed to evaluate the time-dependent in-vivo biocompatibility and mechanichal properties of crosslinked macroporous pure woven collagen meshes (CollaMesh) and compare outcomes to those of Prolene and XenMatrix™ in a rat subcutaneous model. Additional, we aimed to evaluate the feasibility and biological response of CollaMesh in rat suburethral model. The biocompatibility of the subcutaneously implanted meshes were evaluated at 2, 8 and 20 weeks. H&E stained samples were scored for host response in accordance with ISO-10993-6. We explored our novel mesh in a pilot short-term sheep study too. We evaluated the amount and alignment of collagen through image analysis of H&E and Picrosirius red stained slides. Immunohistochemical staining of explanted samples was used to corroborate biocompatibility scoring. To evaluate the mechanical properties of meshes, samples were explanted at 8 and 20 weeks and tested in tension until failure. The stress to failure (strength), strain to failure (elasticity) and modulus (stiffness) of meshes were computed from test data and morphology measurements. Healthy rectus