الفهرس 
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Abstract
A survey to screen bovine tuberculosis (BTB) in dairy cows in some cattle farms in state of Kuwait was carried out, For this purpose, a total of 3086 apparently healthy lactating cows was investigated. These included 2692 from Al-Sulaibya Area (700 from El-shawaaf, 276 from Al-Anizy, 203 from PAAF, 604 Al-Wataiya and 909 from Naif farms), 104 from El-Abdally Area (104 from El-mealy farm) and 290 from Al-Wafra Area (290 from Al-Yasimeen farm) in state of Kuwait were tested by single intradermal tuberculin test (SID) and single intradermal comparative tuberculin test (SIDC). The results revealed that the overall prevalence of bovine tuberculosis were 69 (2.23%) and 23 (0.74%) as determined by SID, and SIDC, respectively. It showed that in Al-Sulaibya Area 69 (2.56%) out of 2692 (50 (7.14%) from El-shawaaf, 3(1.08%) from Al-Anizy, 4(1.97%) from PAAF, 0(0%) from Al-Wataiya and12(1.32%) from Naif farms) were positive by SID, however, all 104 cattle from El-mealy farm (El-Abdally Area 104) and 290 from Al-Yasimeen farm (Al-Wafra Area) were negative by SID. While only 23 (3.23%) out of 700 cattle from El-shawaaf (Al-Sulaibya Area) were positive by SIDC. In the this study, ELISA was carried out for diagnosis of bovine tuberculosis on 90 blood samples from lactating cows, and revealed that the overall prevalence was 13 (14.44) as determined by ELISA. When the ELISA results compared with the results of same 90 lactating cattle by SID and SIDC. It found that overall prevalence as determined by ELISA versus SID and SIDC were 14.4% versus 5% and 1.1%. Furthermore, interferon gamma assay was applied on 161 blood samples taken from cattle of endemic farm and compared its results with the results of SID and SIDC tests. It revealed that 16 (9.9%) out of all 161 samples were positive by interferon gamma assay, whereas, 50 (31%) and 23 (14.3%) out of all 161 cattle were reactive by of SID and SIDC tests, respectively. It could be concluded that BTB was prevalent among some examined lactating cow farms in Kuwait. This illustrated that the important role of these lactating cattle in disseminating and transmit and disseminate of such utmost zoonotic disease in the examined areas, In the present study, SIDC showed lower results than SID. This indicated that SIDC more specific in diagnosis of bovine tuberculosis than SID. ELISA showed higher sensitivities and specificities in diagnosis of BTB than SID and SIDC tests, and interferon gamma assay showed higher sensitivity than skin tests but less specific. The public health importance as well as the preventive measures which would be strictly applied for combating bovine tuberculosis were fully discussed.