الفهرس 
Introduction
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Abstract
H. pylori is a zoonotic bacteria that can be transmitted from cows and buffaloes to human through milk and fecal matter. The current study aimed to detect H. pylori in milk, fecal matter and abomasal content of cows and buffaloes in Assiut Governorate. Moreover, this study was carried out to determine the occurrence of H. pylori among adults suffering from upper GIT symptoms.Milk and fecal swabs were collected from dairy cows (n=79) and buffaloes (n=30) and cultured on Columbia blood agar. H. pylori was isolated from 4 (5.1%) and 3 (10%) of the examined milk samples from dairy cows and buffaloes, respectively. The isolation rate from fecal samples examined from the same animals was 5.1% and 10% of cows and buffaloes, respectively.
The results were confirmed by using PCR and the occurrence was 2.5% and 6.7% in milk of cows and buffaloes, respectively, however, theoccurrence of H. pylori in fecal swabs of cows and buffaloes was 3.8% and 6.7%, respectively.
In addition, fecal swabs and abomasal content swabs were collected from 74 beef cows and 36 beef buffaloes and examined by culture method. H. pylori was isolated from 1(1.4%) of both fecal swabs and abomasal content swabs collected from cows.
In this study we also, collected 49 antral biopsies from patients (group A) suffering from upper GIT symptoms and by upper endoscopy they showed gastritis or gastric ulcer. The patients were recruited from Tropical Medicine and Gastroenterology Department and Endoscopy Unit of Assiut University Hospital. Histological examination by using Giemsa stain revealed that, 9 (18.4%) out of the 49 patients were H. pylori positive. Fecal swabs from the same patients (group A) were taken to be examined by the HPSA test and 14 (28.6%) patients were H. pylori positive from which 7 (14.3) patients were histologically positive.Also, fecal swabs only were collected from other 37 patients (group B) to be examined only by HPSA test and 13 (35.14%) patients were HPSA positive. The net result of these finding concluded that the overall occurrence of H. pylori was33.7% in the examined patients in Assiut Governorate. The occurrence of H. pylori was lower in males (29.73%) than in females (36.73%). Occurrence of H. pylori in different age groups 21-30, 31-40, 41-50, 51-60 and 61-70 was 33.3%, 18.75%, 32.14%, 42.86% and 0%, respectively. The occurrence of H. pylori was higher in rural patients than urban ones (36.92% and 23.8%, respectively). It is clear evident that, the detection of H. pylori was higher in patients with gastritis than in patients with gastric ulcer (34.88% and 16.67%, respectively). All these previous factors (age, sex and residence) were statistically non-significant.