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Abstract This study was performed to evaluate the effect of β-glucan on humoral, cellular immune responses in chickens which vaccinated with killed Salmonella enteritidis (SE) vaccine 0.5 cm S/C at neck region and with live intermediate infectious bursal disease vaccine intra-occulary at 12 days old .One day old 150 Saso chicks were divided into five equal groups, group (1) kept as normal control, group (2) vaccinated with SE vaccine, group (3) vaccinated with IBD vaccine, group (4) supplemented with β-glucan 0.3gm/L water from day one till the end of the experiment and vaccinated with SE vaccine and group (5) supplemented with β-glucan and vaccinated with IBD vaccine. At 26 days old group 2&4 were challenged with SE strain 5.5×108cfu by oral gavage and group 3&5 were challenged with virulent IBDV strain (105.5 EID50/ 0.1 ml) intra-occulary. Blood samples were collected at 23, 33, 40 and 47 days old. The results showed that β-glucan supplementation lead to heterophilia, lymphocytosis and monocytosis with increased Ab titre against Newcastle virus, enhanced phagocytic activity and lead to significant increase in serum IL6 and TNF-α level with decreased IL10 level after challenge. Results revealed that β-glucan increase resistance of chicks against infectious diseases. |