الفهرس 
cover englishOnly 14 pages are availabe for public view
 |  | from | 132 |  |  |
| | | from | 132 | | |
Abstract
Coccidiosis in chickens is one of the major problems of poultry industry that is caused by protozoan parasites of genus Eimeria. Cecal contents and cecal mucosal scraping samples were collected from 50 naturally infected native broiler chicken aging 25 days. The post mortem examination showed hemorrhagic mucosa with bloody cecal core in two cecai. Eimeria oocysts were isolated by single oocyst technique. The obtained sporulated oocysts were identified according to morphological features, and the calculated mean oocyst shape index of randomly 50 oocysts (1.22 μm), mean length (25.2 μm) with SD (±2.24 μm), and mean width (20.6 μm) with SD (±1.6μm) as E. tenella. Species-specific polymerase chain reaction (PCR) test targeting the internal transcribed spacer-1 (ITS-1) sequences of the genomic rDNA using primer pairs (E. tenella ET, E. maxima Ema and Emu and E. nexatrix EN) was performed for isolated Eimeria species. The results of species-specific PCR assays confirmed the presence of E. tenella as it has the characteristic in all of the tested samples bp of 278 at common optimum annealing temperature for this species was determined to be 58 °C but it didn’t show any band with other previous mentioned primer pairs. Nucleotide sequences of Egyptian Eimeria tenella isolate obtained by sequencing of the PCR products from Eimeria species has been entered into the GenBank sequence database under accession number MF537631 and analysis of the obtained sequence revealed high identities 99.6% between Egyptian isolates and the other selective ones. Histopathological evidence showed leakage of blood, oedema and necrosis of cecal mucosa. Eimeria tenella developmental stages in cecum were also detected histopathologically.