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العنوان
In vitro Evaluation of some Fig Cultivars under Salinity Stress /
المؤلف
Elazab, Rana Mohebeldin Saad.
هيئة الاعداد
باحث / رانا محب الدين سعد العزب
مشرف / طاهر احمد يحى
مشرف / عبده محمد عبد اللطيف
مناقش / عزت محمد محمود الفخرانى
مناقش / ماجدة محمود خطاب
الموضوع
Fig. Salinization. Plants--Adaptation.
تاريخ النشر
2018.
عدد الصفحات
3, 73 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
1/1/2018
مكان الإجازة
جامعة القاهرة - كلية الزراعة - الفاكهة
الفهرس
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Abstract

The present investigation was carried out at the Biotechnology Laboratory of Pomology Dept., National Research Center, Dokki, Giza, Egypt during the period from 2015 to 2017 to study plant propagation of four fig cultivars (Black Mission, Aboudy, Conadria and Sultani)by using shoot tip and different concentrations of BAP 0.5 ,2 , 4 mg L-1 and Then put this four cultivars into medium supplemented with different concentrations of NaCl to evaluate the effect of Sodium Chloride on in vitro regenerated fig cultivars. NaCl concentrations (1000, 2000, 3000, 4000, 5000, 6000 and 7000 mg L-1) were used for salinity stress. Sultani fig cultivar from indirect regeneration was cultured on Murashige and Skoog medium containing different NaCl concentrations (0, 2000,4000,6000, 8000and10000mgL-1) were studied. Plant length leaf number, chlorophyll, proline and leaf mineral contents were determined. . The obtained data indicated that Murashig & Skoog medium (MS) supplementedwith0.5 mg/l is most suitable for increasing shoot length, number of leaves, number of branches and greening All fig cultivarswere negatively affected by salinity with different levels. The addition of NaCl significantly decreased plantlet growth, leaf content of N, P, K and total chlorophyll in all cultivars. Cl- and Na+ as well as proline level increased as increasing NaCl concentrations up to 6000 mg L-1NaCl at7000 mg L-1 considered as the lethal dose as it caused high mortality rate of all fig cultivars under study. Sultani fig cultivar accumulated more content of N, P, and K and had higher chlorophyll and proline under high salt stress compared with other figcultivars.Increasing NaCl concentration more than 8000 ppm induced a bad effect on all growth parameters under study up to the lethal concentration by 10000 ppm which caused almost death of the in vitro Regenerated plantlets from subculture1 and subculture 2 of Sultani fig cultivar.