الفهرس 
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Abstract
Hepatitis C virus (HCV) is a universal health problem. HCV infection may proceed to liver fibrosis, cirrhosis, and eventually hepatocellular carcinoma (HCC). The latest is the third leading global cause of cancer-related mortality. Cytokines including IL-27 and TNF-α play a major role as a link between innate and adaptive immunity which in turn deduct the outcome of HCV infection.The present study examined the role of both (-964 A/G) SNP of IL-27p28 rs153109 and (-308 G/A) SNP of TNF-α rs1800629 on the progression of HCV infection in genotype 4a infected patients. Studied HCV infected patients were divided into 38 fibrotic patients, 51 cirrhotic patients and 29 HCC patients. 16 healthy volunteers were used as controls. IL-27p28 rs153109 and TNF-α rs1800629 genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP).
There was no statistically significant difference between the studied groups and IL-27 Genotypes of the studied polymorphism. However, with reference to TNF-α studied polymorphism, there was a significant difference between the HCC and fibrosis group (p = 0.00), and also the cirrhosis and fibrosis group (p = 0.031) revealing that AA genotype is the genotype of risk. Furthermore, the association found between allele frequencies of two studied SNPs and the four studied groups were non-significant. IL-27p28 rs153109 polymorphism may not contribute to HCV progression while TNF-α rs1800629 polymorphism supposed to be a genetic-susceptibility factor for HCV related cirrhosis and HCC, which could contribute to the prevention and early diagnosis of HCV complications.