الفهرس 
Parkinson’s diseaseOnly 14 pages are availabe for public view
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Abstract
The present study was performed to investigate the possible beneficial effects of different doses of Silymarin on experimentally-induced rat model of PD using rotenone in adult male albino rats
The present work was carried out on 60 adult male Albino rats divided into three main groups as follows:
group I: Control group:
This group consists of 10 rats which received no injection and were left untreated for the whole duration of the experiment.
group II: (Positive control): This group consists of 10 rats which were treated with intraperitoneal (i.p.) injection of silymarin (SM) at dose of 200 mg/kg, once daily for 2 weeks.
group III: (Rotenone-treated):
This group consists of 40 rats which were treated with subcutaneous (sc) injection of rotenone at dose of 1.5 mg/kg/48h for 12 days. At the end of the experiment, the rats of this group were subjected to preliminary behavioral tests and the rats that showed
PD features were randomly subdivided into 4 equal groups of 10 rats each:
group IIIa: (Rotenone-treated):
The rats of this group did not receive any further treatment.
Summary & Conclusion
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group IIIb: (100 mg/Kg SM-RT):
The rats of this group were treated with i.p. administration of silymarin at dose of 100 mg/kg, once daily for 2 weeks.
group IIIc: (200 mg/Kg SM-RT):
The rats of this group were treated with i.p. administration of silymarin at dose of 200 mg/kg, once daily for 2 weeks.
group IIId: (300 mg/Kg SM-RT):
The rats of this group were treated with i.p. administration of silymarin at dose of 300 mg/kg, once daily for 2 weeks.
At the end of the experiment, the following behavioral tests were carried out on all groups. Rats were trained on these tests for three days, and then the tests were carried out:
1-Bar test for catalepsy.
2-Forepaw stride length to assess the shuffling gate observed in PD patients.
Retro-orbital blood samples were collected from all animals for serum preparation. Then, all animals were sacrificed, and brains were dissected for preparation of brain tissue homogenate. The following parameters were measured in prepared sera for all groups:
1-Serum oxidative DNA damage (8-hydroxy-2’-deoxyguanosine level).
The following parameters were measured in prepared brain tissue homogenate