الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
Occlusal pits and fissures are the most susceptible sites for the development of dental caries. Pit and fissure sealants were produced to prevent occlusal caries because they act as physical barriers between the oral environment and the tooth surface. The longevity and effectiveness of restorations also depend on the interaction between microorganisms and restorative materials.
This in-vitro study was conducted to compare between the effect of two fluoride releasing fissure sealants (Teethmate F-1 and Fissurit F) on the inhibition of Streptococcus mutans and Lactobacillus acidophilus.
The strains of Streptococcus mutans were isolated in Mitis Salivarius Bacitracin agar and the strains of Lactobacillus acidophilus were isolated in Rogosa selective agar plate. The agar plates were incubated for 48 hours at 37°C. The strains were grown in the Brain Heart Infusion broth for Streptococcus mutans and in the MRS broth for Lactobacillus acidophilus and were incubated anaerobically for 18 hours at 37°C. The strains were grown and subcultured in Mitis Salivarius agar for Streptococcus mutans and in blood agar plates for Lactobacillus acidophilus. Four wells were prepared on each agar plate using a standard bore with a diameter of 5 mm. Ten specimens of each material were prepared with the diameter of 5 mm and height of 3 mm and placed in the agar plates (Kumar et al.,
The agar plates were further incubated anaerobically for 48 hours at 37°C. The antimicrobial property of materials were assessed, which appeared from the circular zones of bacterial inhibition around each material. The diameter of these zones of bacterial inhibition were measured in millimeters after 48 hours and 72 hours of incubation. Data obtained were statistically analyzed by Kolmogorov-Smirnov and Shapiro-Wilk, Mann- Whitney and Wilcoxon signed-rank tests. The significance level was set at P ≤ 0.05.
The results showed that Teethmate F-1 showed statistically significant higher mean inhibition zone diameter than Fissurit F on Streptococcus mutans after both 48 hours and 72 hours of application. On Lactobacillus acidophilus after both 48 hours and 72 hours of application; there was no statistically significant difference between the two groups. The findings on both Streptococcus mutans and Lactobacillus acidophilus showed that for Teethmate F-1 group; there was non- statistically significant increase in mean inhibition zone diameter after 72 hours. While for Fissurit F group; there was a statistically significant increase in mean inhibition zone diameter after 72 hours.