الفهرس 
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Abstract
In this study, a total of (160) marine fishes of two different species represented as: seabass
(Dicentrarchus labrax) and Tilapia zillii randomly collected from Lake Temsah at Ismailia
governorate from August 2015 to July 2016. 76 fishes were founded to be infected with
both V. anguillarum and V. parahemolyticus, as Gram negative, short rod bacilli as comma
shaped and motile bacteria. Dicentrarchus labrax was the most infected fish spp (41%),
pursued by Tilapia zillii (35%). The highest prevalence of vibriosis among naturally
infected marine fishes was recorded during summer (68%), pursued by spring (55%), then
autumn (40%), while the minimal prevalence (28%) was registered during winter
Molecular investigation of pure isolates using sets of both universal and specific primers
gave PCR products with clear band of 120 and 519 bp lengths, respectively.
Experimentally infected fishes subjected to higher dose (107 ) of V. anguillarum presented
higher morbidity and mortality rate (up to 80%). Most of infected fishes showed skin
ulceration in the ocular side, others displayed redness and scattered hemorrhagic spots on
the abdomen with fin erosion. The histopathology of naturally infected marine fishes
exhibited severe inflammatory reactions manifested by leukocytes infiltration with
activation of melanomacrophage centers as well as tissue necrosis..