الفهرس 
Acknowledgement
Olive oil and its effectsOnly 14 pages are availabe for public view
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Abstract
The current study was designed to evaluate the efficacy of olive oil as an antimutagenic agent in imidacloprid toxicity through determination of DNA damage, micronucleus frequency and chromosomal aberrations in bone marrow in relation to biochemical parameters as malondialdehyde and glutathione peroxidase in serum. Two hundred male albino rats were divided into 4 groups (50 each). group 1(G1) was used as a negative control group. group II (G2) was exposed to 22.5 mg/kg b. w. of IMI (1/20 LD50) orally using stomach tube day by day for 4, 6, 8, 10 and 12 weeks as a positive control. group III (G3) was treated with olive oil in a dose of 10 ml/kg b. w. orally using stomach tube day by day for two weeks before 4, 6, 8, 10 and 12 weeks of IMI exposure. group IV (G4) was treated with olive oil in a dose of 10 ml/kg b. w. orally using stomach tube day by day for two weeks after 4, 6, 8, 10 and 12 weeks of IMI exposure. At end of treatment for each group, 10 rats were sacrificed by cervical dislocation for bone marrow and blood collection. The investigated parameters were DNA damage, micronucleus frequency, chromosomal aberrations and biochemical parameters (malondialdehyde and glutathione peroxidase).
The obtained results of comet assay showed a significant increase in TL, tail DNA%, TM, and OTM in G2 in comparison with the control group at 6, 8, 10 and 12th weeks for TL, tail DNA% and TM and at 6, 8 and 12th weeks for OTM. These parameters significantly decreased in G3 in comparison with G2 at 6, 8, 10 and 12th weeks for TL and tail DNA% and at 8, 10 and 12th weeks for TM and at 8 and 12th weeks for OTM. Also, a significant decrease was observed in G4 in comparison with G2 at 6, 8 and 12th weeks for TL and at 12th week for tail DNA% and at 8 and 10th weeks for TM and at 8 and 12th weeks for OTM. Moreover, TL and tail DNA % significantly decreased in G3 in comparison with G4 at 10th week for TL and at 8th week for tail DNA%. The obtained results of chromosomal aberrations assay revealed the percentage of normal metaphase cells significantly decreased and the percentage of metaphase cells with chromosomal aberrations significantly increased in G2 in comparison with control group at 6, 8, 10 and 12th weeks.
The percentage of normal metaphase cells significantly increased and the percentage of metaphase cells with chromosomal aberrations significantly decreased in G3 in comparison with G2 at 4, 6, 8, 10 and 12th weeks.
A significant increase was recorded in the percentage of normal metaphase cells and the percentage of metaphase cells with chromosomal aberrations significantly decreased in G4 in comparison with G2 at 4, 8, and 12th weeks. The percentage of normal metaphase cells significantly increased and the percentage of metaphase cells with chromosomal aberrations significantly decreased in G3 when compared with G4 at 6 and 10th weeks. The percentage of normal metaphase cells significantly increased and the percentage of aberrant cells significantly decreased in G4 when compared with G3 at 8th week. Micronucleus assay results revealed the frequency of micronucleated polychromatic erythrocytes significantly increased in G2 in comparison with control group (G1) at 6, 8, 10 and 12th weeks while frequency of MnPCEs significantly decreased in G3 when compared with G2 and G4 at 6th week also a significant decrease was recorded in G4 when compared with G2 at 8th weeks. The ratio of polychromatic to normochromatic erythrocytes showed a significant decrease in G2 in comparison with the control group at 8, 10 and 12th weeks also the percentage of polychromatic erythrocytes showed a significant decrease in G2 when compared with control group at 8, 10 and 12th weeks. A significant increase in the percentage of PCEs was recorded in G3 at 8th week and in G4 at the 12th week when compared with G2. Malondialdehyde level showed a significant increase in G2 in comparison with the control group over the whole period of the experiment. A significant decrease was recorded in G3 and G4 when compared with G2 at 6, 8 and 12th weeks.
Glutathione peroxidase activity showed a significant decrease in G2 all over the experiment period in comparison with control but G3 and G4 showed a significant increase all over the experiment period when compared with G2 also a significant increase was recorded in G3 at 6, 10 and 12th weeks when compared with G4.