الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 223 |  |  |
| | | from | 223 | | |
Abstract
Carcinogenesis is a complex process that can involve various modifications to a number of molecular pathways as well as genetic alterations, and ultimately leads to malignant transformation and HCC disease progression. Several studies demonstrated the toxicity of BPA even at low doses, by causing injury in the liver, kidney, and brain and other organs in rodents by forming reactive oxygen species (ROS).Cisplatin is extensively used as a chemotherapeutic agent for the treatment of HCC. A major problem with CIS treatment of HCC is the development of CIS chemoresistanceIn this study adult male Wister albino rats weighing (100-120g) were used, they were divided into seven groups, 6 rats in each group as follow : Group-1-(C) : rats of this group received no treatment. Group-2-(CO): a vehicle control group, rats of this group was orally administrated day by day with 1ml (0.1ml DMSO + 0.9ml corn oil)/kg.bw for 15 weeks. Group-3-(BPA) : rats of this group were orally, day by day gavaged with bisphenol A (322mg/kg.bw) dissolved in (0.1ml DMSO + 0.9ml corn oil) for 15 weeks. Group-4-(HCC): rats of this group treated with a single intraperitoneal injection of freshly prepared DENA (200 mg/kg.bw), then two weeks later, they received subcutaneous injection of CCl4 once every week (3ml/kg.bw)for 10 weeks. Group-5-(HCC+BPA) : rats of this group treated with the same treatment of group 3 and 4. Group-6-(HCC+CIS) : rats of this group received the same treatment as described in group 4 and after 12 weeks received an intraperitoneal injection of cisplatin (1.5mg/kg.bw) repeated twice a week for 3 weeks. Group-7-(HCC+BPA+CIS) : rats of this group received the same treatment as described in group 5 and at the last 3 weeks of the 15 week received an intraperitoneal injection of cisplatin (1.5mg/kg.bw) repeated twice a week. Our study revealed a significant increase in the liver marker enzymes, AST, ALT, ALP and total bilirubin level in the serum while a significant decrease in total protein and albumin levels were recorded in BPA, HCC and HCC + BPA groups. In parallel, marked elevations in the levels of MDA and H2O2 and in contrarily a reduction occurs in GSH level, CAT, SOD and GST activities in the liver of rats.Also, results showed a significant increases in serum TL, TC, TG, LDL-C and VLDL-C levels; but a significant decrease in serum HDL-C level, were observed in BPA, HCC and HCC + BPA groups. Furthermore an elevation in both AFP and TNF-α were recorded in the three groups. Moreover, BPA, HCC as well as HCC+BPA group recorded an elevation in the apoptosis process in the rats’ livers as manifested by significant increases in hepatic p53 and caspase-3 levels, and a decrease in Bcl2 level. The treatment of the two rats groups HCC and HCC+BPA with cisplatin significantly reduced the adverse changes in the measured parameters of liver function enzymes, oxidative stress, antioxidants, lipid profile and apoptosis, when we compared the first treatment group to the HCC one and the second treatment group with HCC+BPA group where it recorded less improvement in all investigated parameters. from the previous data, this study warning from the dangerous effect of bisphenol (A) on the liver, especially in case of patients suffering from hepatocellular carcinoma and those treated by cisplatin chemotherapy, where the resistance to the treatment was recorded.