الفهرس 
Nature of HCVOnly 14 pages are availabe for public view
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Abstract
Summary
CHC is a serious national problem in Egypt. Egypt has the highest prevalence CHC infection in the world. Host genetic diversity was believed to contribute to the susceptibility to HCV infection through host immune response to HCV.
This study was aimed to assess the association between genetic polymorphisms of TLRs (3, 7 and 8) and susceptibility to CHC infection in Egyptian patients.
This was achieved through 2758 subjects. The subjects of the study were classified into 3 distinctive groups according to HCV infection state. The first group is 1310 of Egyptian subjects with CHC infected. The second group includes 1340 HCV negative. The last group is 108 of SVC subjects.
Written informed consent was obtained from each patient included in the study, and the study protocol conforms to the ethical guidelines of the 1975 Declaration of Helsinki as reflected in a priori approval by the institution’s human research committee in Mansoura University.
All subjects were assessed through medical history and laboratory investigations were evaluated. ANA , Serum Albumin, Serum Bilirubin, ALT, AST, ALP, Serum creatinine, anti HCV antibodies, HCV-RNA, HBV surface antigen, HIV antibodies and real time-PCR of TLR3 (rs3775290, rs3775291and rs5743312), TLR7 (rs3853839 and rs179008) and TLR8 (rs3764880) was performed.
All populations of the study are Hardy-Weinberg equilibrium, except the males with regards to TLR7 and TLR8. ALL SNPs of this study are polymorphic enough for genetic analysis in study population.
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Results:
The C allele of TLR3 (rs3775290, rs3775291and rs5743312) C/T polymorphism is found to be significantly associated with negative control group when compared to CHC group and with SVC group as compared to CHC group (P<0.05). While it is not significantly associated to negative group when compared to SVC group (P value is not applicable).
The C allele of TLR7 rs3853839 (C/G) polymorphism is found to be significantly associated with negative control group when compared to CHC group (P<0.05) in both gender subpopulation. AS well as it is significantly associated with SVC group as compared to CHC group (P<0.05) in female gender subpopulation. While it is not significantly associated to negative control group when compared to SVC group in both gender subpopulation (P value is 0.18180 and value is not applicable) in female and male, respectively.
The T allele of TLR7 rs179008 (A/T) polymorphism is found to be significantly associated with CHC group when compared to negative control group (P<0.05) in both gender subpopulation. AS well as it is significantly associated with CHC infected group as compared to SVC group (P<0.05) in female gender subpopulation. While it is not significantly associated to SVC group when compared to negative control (P value is not applicable) in both gender subpopulation.
The A allele of TLR8 rs3764880 (A/G) polymorphism is found to be significantly associated with CHC group when compared to negative control group and SVC group (P<0.05) in both gender subpopulation. And it is significantly
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associated to SVC group when compared to negative control (P<0.05) in female gender subpopulation.