الفهرس | Only 14 pages are availabe for public view |
Abstract Fifty random samples of raw milk were collected from different dairy farms and dairy shops in Sharkia Govemorate, Egypt. A survey was conducted to determine the hygienic quality of the examined raw milk samples and public health hazards by applying bacteriological examination and antibiotic resistance of S. aureus to different antibiotics. Also the inhibitory effect of L. rhamnosus (ATCC 53103) on S. aureus was determined. Staphylococcus spp. was detected in all dairy farms and dairy shops raw milk samples with mean values of 7.04xl05 ±0.26xI05 cfu/ml and 7.64x105 ±O.l5x105 cfu/ml, respectively. S. aureus, S. epidermidis and S. Saprophyticus were isolated from dairy farms raw milk samples with percentages of 52.00%, 28.00% and 20.00%, respectively. While the same strains were isolated from dairy shops raw milk samples with percentages of 76.00%, 16.00% and 8.00%, respectively. Ten S. aureus isolates were identified by using MALDI- TOF MS. (Vitek MS) . Resistance profile of ten S.aureus isolates (5 each of dairy farms and dairy shops raw milk samples ) were examined by using of the Vitek 2 system against Nitrofurantoin, Ciprofloxacin, Gentamycin, Levofloxacin, QuinupristinlDalfopristin, Linezolide, Vancomycin, Oxacilline, Moxifloxacin, Erythromycin, Clindamycin, Tetracycline and Pencillin. It was found that the drug of choice for S. aureus infection Treatment was Nitrofurantoin. |