الفهرس 
Chapter 1:Acute kidney injuryOnly 14 pages are availabe for public view
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Abstract
To date, no effective therapies are at hand to prevent or treat AKI specifically. Stem cells are primal cells which are considered to be progenitor of more than 200 cell types present in adult body. Much less is known about the effect of haematopoietic stem cells (HSCs), the other major fraction of the BMSC, in AKI.
The aim of the present work was to study the effect of CD34+ HSC/HPC (haematopoietic stem cell/progenitor cell)separated from human umbilical cord blood in the treatment of AKI in experimental rats induced by sepsis.
Thirty male Swiss albino rats, weighing approximately 150- 250g were used in the present study.Rats were maintained under controlled temperature, humidity, and 12 hour light/dark cycles. The animals were fed standard rodent chow and allowed free access to water and libitum, and were kept for 10 days prior to any procedure to allow proper acclimatization.
Animals were randomly divided-into-three groups: group I: Control group (10 rats). group II: AKI non treated group (10 rats) Sepsis-induced AKI rat model were used. group III: Stem cell- AKI treated group (10 rats). Rats with established acute kidney injury were injected with CD34+ HSC/HPC (haematopoietic stem cell/progenitor cell) separated from human umbilical cord blood. In the experimental groups serum creatinine, serum urea, blood urea nitrogen , cytatin C, serum rat transforming growth factor beta ( Rat TGF-β),serum rat Interleukin 18 ( Rat IL-18), serum rat Interleukin 6(Rat IL-6),serum rat Nitric Oxide (Rat NO),serum rat total antioxidant capacity( Rat TAC), rat renal blood flow velocity and rat resistance parameter (Rat RP) were assessed .
Our results summarized that:
In this study, Serum creatinine level was significantly higher in AKI group in comparison with both control group and stem cell treated group(P=0.001)while no statistically significant differene was found between control group and stem cell treated group regarding serum creatinine level (P= 0.086)
Serum urea level was significantly higher in AKI group in comparison with both control group and stem cell treated group( P=0.001) while no statistically significant differene was found between control group and stem cell treated group regarding serum urea level(P= 0.257)
Blood urea nitrogen was significantly higher in AKI group in comparison with both control group and stem cell treated group ( P=0.001) and also high significant differene was found between control group and stem cell treated group regarding serum urea level(P:0.001)
serum cystatin C was significantly higher in AKI group in comparison with both control group and stem cell treated group ( P=0.001) while no statistically significant differene was found between control group and stem cell treated group regarding serum serum cystatin C (P= 0.944)
Serum TGF-β was significantly higher in AKI group in comparison with both control group and stem cell treated group ( P=0.001) and also high significant differene was found between control group and stem cell treated group regarding serum TGF-β (P= 0.001)
Serum IL-18 was significantly higher in AKI group in comparison with both control group and stem cell treated group ( P=0.001) and also high significant differene was found between control group and stem cell treated group regarding Serum IL-18(P= 0.002)
Serum IL-6 was significantly higher in AKI group in comparison with both control group and stem cell treated group ( P=0.001) while no statistically significant differene was found between control group and stem cell treated group regarding Serum IL-6(P= 0.070)
Serum NO was significantly higher in AKI group in comparison with both control group and stem cell treated group ( P=0.001) while no statistically significant differene was found between control group and stem cell treated group regarding Serum NO(P= 0.539)
Serum TAC was significantly higher in AKI group in comparison with both control group and stem cell treated group ( P=0.001) and also significant differene was found between control group and stem cell treated group regarding Serum TAC(P= 0.013)
Renal artery doppler Flow velocity was significantly higher in AKI group in comparison with both control group and stem cell treated group ( P=0.001) while no statistically significant differene was found between control group and stem cell treated group regarding Renal artery doppler Flow velocity (P= 0.383)
RP was significantly higher in AKI group in comparison with both control group and stem cell treated group ( P=0.001) while no statistically significant differene was found between control group and stem cell treated group regarding RP(P= 0.144)
Immuno-histochemical studies results showed that. -stained sections, from acute kidney injury group showed negative staining for the monoclonal mouse Anti-human CD34.
- stained sections, from AKI-stem cell-treated group showed a positive staining (golden staingolden staingolden staingolden staingolden staingolden staingolden stain golden staingolden staingolden staingolden stain ) of the renal tubules for the monoclonal mouse Anti-human CD34.