الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
”Guillain-Barré syndrome” is a rare, however serious autoimmune peripheral neuropathy, in which the immune system attacks healthy nerve cells in peripheral nervous system.
The precise etiology of GBS is unknown; however it is usually triggered by an associated infectious illness such as Campylobacter jejuni infection, Influenza, Cytomegalovirus, Epstein-Barr virus infection, Mycoplasma pneumonia, HIV or AIDS.
One third of the post infectious GBS are due to viral causes. CMV, HHV-6 and EBV are members of the herpes virus family that are implicated in pathogenesis of GBS. It is believed that in GBS patients, infections with cytomegalovirus (CMV) accounts for 13%, Epestein Barr virus (EBV) 10% and Human herpes virus-6 accounts for 1%.
The diagnosis of GBS can be usually made by clinical examination and by nerve conduction studies in the majority of patients. Molecular techniques are easy and rapid methods used to confirm the etiological diagnosis of GBS and are rarely ordered by the attending clinician. Thus, little data is available about the infectious etiology of the disease that may be helpful in treatment and estimating the disease pathogenesis.
This study is novel in determining the association of GBS with not only the presence of viral DNA in plasma of GBS patients but also the correlation between viral load and severity of the disease.
The study was performed on thirty patients diagnosed as Guillain Barre Syndrome according to Brighton criteria and twenty control subjects, apparently healthy persons matched with age and sex. Multiplex real time PCR was performed after extraction of viral DNA to detect gene sequences (cytomegalovirus UL 32, HHV 6 U67 and EBV BHRF1)
Interestingly, our study revealed that CMV UL 32 gene presence was higher among studied cases as compared to controls but results of HHV 6 U67 and EBV BHRF1genes were not significant. We could not prove a significant correlation between viral load and severity of the disease due to limited number of studied cases.
CONCLUSION
from the study we can conclude that: