الفهرس | Only 14 pages are availabe for public view |
Abstract Acinetobacter species are non-motile, oxidase-negative, Gram-negative bacteria frequently isolated in nosocomial infections due to their ability to survive in a hospital milieu and to persist for extended periods of time on surfaces. Acinetobacter spp. have been implicated as the cause of serious infections such as ventilator-associated pneumonia (VAP), urinary tract infection, wound infection and septicemia. A. baumannii exhibits a remarkable ability to rapidly develop antibiotic resistance that led to multidrug resistance (MDR) within a few decades. The aim of this study is to detect the prevalence of Acinetobacter baumannii in patients of different Intensive Care Units, to find out the antimicrobial susceptibility of the isolated organism using disc diffusion method, and to detect some of the genes responsible for the antimicrobial resistance among the isolated strains. Also to determine the aminoglycoside resistance patterns in Acinetobacter species obtained from clinical specimens and to identify the genetic determinants responsible for aminoglycoside resistance including inactivating enzymes and rRNA methylases. In conclusion • High level of antibiotic resistance including multidrug resistance isolates were detected among Acinetobacter baumannii isolated clinical samples. • High level of aminoglycoside antibiotic resistance was detected among Acinetobacter baumannii isolates. • Among the 100 Acinetobacter baumannii isolates, resistant to kanamycin and neomycin was (100%) followed by amikacin (96%), gentamicin and netilmicin (92%) each while resistance to tobramycin was (87%). • Aminoglycoside modified enzymes genes were detected in the following order, aphA6, armA, aphA1, aacA4, aadA1, aacC1, aadB, aacC2 and rmtB. • 16S rRNA methylase was detected in 83% of the isolates. • The results of this study showed a significant prevalence of genes encoding aminoglycosides modifying enzymes in Acinetobacter baumannii isolates in the studied region. Therefore, it is important to be concern about the extensive monitoring of antibiotic resistance and the prevention of the emergence of multi drug resistance strains. |