الفهرس 
TitleOnly 14 pages are availabe for public view
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Abstract
Acinetobacter species are non-motile, oxidase-negative, Gram-negative bacteria
frequently isolated in nosocomial infections due to their ability to survive in a hospital
milieu and to persist for extended periods of time on surfaces.
Acinetobacter spp. have been implicated as the cause of serious infections such as
ventilator-associated pneumonia (VAP), urinary tract infection, wound infection and
septicemia. A. baumannii exhibits a remarkable ability to rapidly develop antibiotic
resistance that led to multidrug resistance (MDR) within a few decades.
The aim of this study is to detect the prevalence of Acinetobacter baumannii in
patients of different Intensive Care Units, to find out the antimicrobial susceptibility of the
isolated organism using disc diffusion method, and to detect some of the genes responsible
for the antimicrobial resistance among the isolated strains. Also to determine the
aminoglycoside resistance patterns in Acinetobacter species obtained from clinical
specimens and to identify the genetic determinants responsible for aminoglycoside
resistance including inactivating enzymes and rRNA methylases.
In conclusion
• High level of antibiotic resistance including multidrug resistance isolates were
detected among Acinetobacter baumannii isolated clinical samples.
• High level of aminoglycoside antibiotic resistance was detected among
Acinetobacter baumannii isolates.
• Among the 100 Acinetobacter baumannii isolates, resistant to kanamycin and
neomycin was (100%) followed by amikacin (96%), gentamicin and netilmicin
(92%) each while resistance to tobramycin was (87%).
• Aminoglycoside modified enzymes genes were detected in the following order,
aphA6, armA, aphA1, aacA4, aadA1, aacC1, aadB, aacC2 and rmtB.
• 16S rRNA methylase was detected in 83% of the isolates.
• The results of this study showed a significant prevalence of genes encoding
aminoglycosides modifying enzymes in Acinetobacter baumannii isolates in the
studied region. Therefore, it is important to be concern about the extensive
monitoring of antibiotic resistance and the prevention of the emergence of multi
drug resistance strains.