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Abstract A total of (281) fecal samples were collected from different species of birds of prey at Giza zoo between September 2016 and December 2017 for isolation and identification of some genuses of Family Enterobacteriacea. E. coli was isolated by ratio (51.1%), Salmonella spp. (1.8%), Proteus spp (30.9%), Shigella spp(5.6%), Enterobacter spp(4.6%), Citrobacter spp(3.2%), and Pseudomonas spp (2.8%). Seasonal prevalence of Escherichia coli infection revealed that the highest incidence occurred in summer season 76.5%. Followed by autumn 54.9%, then in spring 46.1%, and finally in winter 34.9%. While all Salmonella isolates were recovered only during winter season. The most prevalent E. coli serogroups were O55 and O63 (16.67%) followed by O151 and O158 (12.5%) then O26, O28a, O148 and untypable (8.33%), followed by O27 and O112 (4.17%) while, All Salmonella strains were found belong to salmonella enterica serovar Typhimurium by ratio 100%. Antibiotic sensitivity testing revealed that the isolated E. coli strains showed high susceptibility rate to Colistine and Apramycin 100%, Gentamycine and Norfloxacine 95.8%, Spectinomycin 91.7%, Nalidixic A. 75%, Sulfamethoxazole70.8%, followed by Cefotriaxone 58.3% and Doxycyclin 54.2%. While the strains showed resistance to some antimicrobial agents mainly Erythromycin and Penicillin G. Based on PCR analysis eaeA virulence gene detected in (10/11) 90.9% of tested E. coli strains, tsh gene found in (2/11) 18.18%, iss gene detected in (3/11) 27.27%, stx1 was not found in any examined E. coli strain (0/11) 0%, while stx2 was found in (4/11)36.36% of the examined E. coli strains. |