الفهرس 
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Abstract
This thesis is devoted to the development of new methods for the analysis of drugs contain asymmetric carbon including certain drugs affecting respiratory system namely; formoterol fumarate (FF), indacaterol maleate (IND), glycopyrronium bromide (GLY), tiotropium bromide (TIO), ciclesonide (CIC), antihistamine namely chloropheniramine maleate (CP), anti-inflammatory and analgesic namely ibuprofen (IBU) and drugs affecting central nervous system namely phenylephrine hydrochloride (PHE) and pseudoephedrine hydrochloride (PSE). A simple and rapid isocratic ion pair chromatographic method (IPC) with UV detection was developed for simultaneous determination of binary mixture; indacaterol maleate and glycopyrronium bromide in their raw materials as well as inhaler capsules. The IPC method was utilized to study the in-vitro drug release as well as content uniformity testing according to USP guidelines. Besides, Derivative, ratio derivative spectrophotometry and IPC methods were introduced for the simultaneous determination of formoterol fumarate and glycopyrronium bromide in their laboratory prepared mixtures and co-formulated metered dose inhaler. Moreover, a sensitive and specific IPC method with ultraviolet detection was developed for determination of formoterol fumarate, tiotropium bromide and ciclesonide in raw materials and in its metered dose inhaler. Additionally, dual-cyclodextrin system (S𝛽CD and 𝛽CD) was utilized as a chiral aqueous mobile phase additive to separate chloropheniramine (CP) enantiomers in the presence of caffeine (CAF) on an achiral column instead of expensive chiral stationary phases. Furthermore, two ternary mixtures used for cold treatment in their co-formulated tablets were simultaneously determined by micellar liquid chromatographic method adopting ultraviolet detection. Finally, first and second derivative emission spectrofluorimetric methods were investigated for the determination of phenylephrine hydrochloride and ibuprofen in their tablets and spiked human plasma. The validation criteria of the developed methods were intensively studied. All the results were statistically analyzed and compared with those given by official or comparison methods.