الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
The objectives of this study are isolation, identification and molecular characterization of MDV circulating in Dakahlia Governorate, Egypt through the following: Trials for isolation of the suspected virus from samples collected from diseased chickens, Serological Identification of MDV in both collected field samples and egg passage samples (third passages) using AGPT and indirect IFA, Molecular identification of MDV using conventional and real time-PCR depending on ICP4 gene and Nucleotide Sequence analysis of amplified ICP4 gene of the isolated MDV nucleic acid. In the present study, 40 samples including 16 feather follicle epithelium, 6 ovaries, 6 kidneys, 6 liver and 6 spleens were collected from 16 diseased chicken farmswere used for isolation of MDV that was carried out .Our results revealed the failure to isolate MDV from 5 field samples. These samples included 1 samples from feather follicle epithelium samples and 2 samples from spleen and liver organs. A white line of precipitin which indicate the positive results in AGPT was observed between known MDV hyperimmune serum and both of 19 field samples and 12 3rd passage samples including 4 (25%) samples from feather follicle epithelium samples and 2 (33.3%) 3rd egg passaged samples from ovary and kidney ,1 (16.6%) 3rd egg passaged samples from spleen and liver .Also, yellowish green color as apositive result for IFAT appeared in 36 frozen field tissues and 32 CAMs prepared sections .All samples submitted to PCR showed positive results of ICP4 gene amplification with correct size. Phylogenetic tree pattern after alignments of the sequenced virus revealed that our isolate in this study was closely related to USA with percent identity of 96.9%.