الفهرس 
تأثير الأستروجينات النباتية على الاداء التناسلى والمعايير الهيماتولوجية و البيوكيميائية فى ذكور الأرانبOnly 14 pages are availabe for public view
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Abstract
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VII- SUMMARY
The present study was aimed to investigate the effects of different levels of soybean isoflavones (Genistein and daidzein) and the source of dietary phytoestrogens (soybean and flaxseed) on: reproductive performance, hemato-biochemical parameters and antioxidant enzyme activities in adult male rabbits. The present study was carried out at the Physiology Rabbit Research Laboratory. Animal and Fish production Department, Faculty of Agriculture, Alexandria University, Egypt, during winter and spring seasons (from January to April 2016). Thirty-five sexually mature V-line rabbit bucks 6-7 months old (average initial body weight 3.000 ± 0.06 kg) at the beginning of the experiment were used. The study was carried out in two experiments: to evaluate the effect of a long term administration of low and high doses of soybean isoflavones and the effect of two dietary phytoestrogens sources (soybean and linseed) on reproductive performance and hemato-biochemical parameters in male rabbits
The first experiment (EXP1) aimed to study the effects of exposure to purified soybean isoflavones on reproductive performance and heamato-biochemical parameters in male rabbits. Twenty- one sexually mature Rabbit bucks were classified into three groups, 7 bucks each. The first group served as control (n=7) and received the basal ration (free phytoestrogens) without any addition. The second group (n=7) received the basal diet plus 5mg/ Kg body weight /every other day of soybean isoflavones (low does, LISO group). While third group (n=7) received the basal diet plus 20 mg/ Kg body weight /day of soybean isoflavones (high does, HISO group).
The second experiment (EXP2) aimed to study the effects of prolonged exposure of dietary phytoestrogens on reproductive performance and heamato-biochemical parameters in male rabbits. Twenty-one sexually mature rabbit bucks for 12 conductive weeks were randomly classified into three homogenous groups, the control group received phytoestrogen free diet. The second group (n=7) received soybean (isflavones source). The third group received linseed (lignan source).
During the experimental period physiological parameters including body weight, feed intake, rectal temperature of animal were recorded weekly. Libido and semen quality
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parameters were weekly evaluated throughout the experimental period. Semen volume, reaction time, progressive motility, concentration of sperm and live and abnormal sperm percentages were recorded. Previous parameters were used to calculate TSO,TMS and TFSF. Seminal plasma was analyzed bi-weekly for total protein and albumin. Initial fructose concentration was determined at the end the treatment. For testing fertility of bucks every buck in each group was mated with 3-4 nulliparous female rabbits. Blood samples were bi-weekly collected during the entire experimental period to assess the hematological parameters and blood plasma total protein, albumin, glucose and cholesterol. Also, blood plasma total antioxidant capacity (TAC), glutathione-S-transferase (GST), superoxide dismutase (SOD) and malondialdehyde (MDA) concentrations and triiodothyronine (T3), testosterone hormones concentrations were measured.
The results of the two studies can be summarized as follows:
1- There were insignificant effects of the different levels of soybean isoflavones (EXP1) or the source of dietary phytoestrogens (EXP2) on rabbit body weight, feed intake and rectal temperature.
2- Treatments with low or high levels of soybean isoflavones (EXP1) or the source of dietary phytoestrogens (EXP2) increased significantly reaction times of rabbit bucks (lower libido) compared to the control rabbit bucks.
3- Ejaculate volume was not affected by with low or high levels of soybean isoflavones (EXP1) or the source of dietary phytoestrogens (EXP2)
4- In the two studies, the sperm concentrations in the semen were decreased by treatment. The percentage of progressive motile sperm was increased ((P> 0.05) by low dose of soybean-isoflavones compared with the control and high dose of soybean-isoflvaones. On the other hand, the percentage of motile sperm was improved by feeding bucks the phytoestrogen-containing diets relative to bucks fed the control diet
5- The percentage of live and abnormal sperm were not affected by different doses of soybean isoflavones. But, using to types of phytoestrogens showed that feeding the SOY increased (P> 0.05) the percentage of live sperm compared with bucks fed the CON diet; whereas, feeding bucks the LIN containing diet resulted in an intermediate value. The
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percentage of abnormal sperm in ejaculates was greater for bucks fed the LIN diet than bucks fed the SOY diet.
6- Rabbit does mated with control bucks or those received low or high dose of soybean-isoflavones (EXP1) or fed different source of phytoestrogens (EXP2) possessed comparable kindling rates , litter sizes, litter weights and litter viabilities at birth
7- The results showed that either low or high doses of soybean isoflavones did not affect most of hematological parameters, except for both of hemoglobin and hematocrit. Thus when two types of phytoestrogens (soy and lignin) the results showed insignificant differences among all studied parameters, except of MCH, WBC, lymphocyte and neutrophils, but the results showed significant differences among treatments in these parameters.
8- Both doses of isoflavones and both sources of dietary phytoestrogens decreased (p> 0.05) blood plasma total protein and albumin concentrations.
9- Rabbit bucks treated with the high dose of isoflavones had lower concentrations of blood plasma MDA and higher concentrations of blood plasma TAC than the control, while such changes were not observed for rabbit bucks treated with the low dose of isoflavones. Concentration of blood plasma MDA was decreased by feeding either the SOY or LIN diets. The LIN diet had the highest blood plasma TAC value, followed by those fed the Soy-containing diet and CON diet.
10- The both levels of soybean isoflavones (EXP1) or source of dietary phytoestrogens increased significantly (p> 0.05) concentrations of blood plasma T3, but decreased concentrations of blood plasma T compared to the control.