الفهرس 
Review of LiteratureOnly 14 pages are availabe for public view
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Abstract
HCC is a major cause of morbidity and mortality; it is the sixth most common cancer worldwide, and the third leading cause of cancer related deaths. Its prognosis is poor and early detection is of great importance. The most common form of genetic variation between individuals is SNP which corresponds to a modification of a DNA sequence due to the change of a single nucleotide.
Epidermal growth factor (EGF) is a mitogen for hepatocytes and plays a critical role in liver tissue regeneration. Mounting evidence supports a role for EGF in malignant transformation, tumor growth and progression. Over-expression of a secreted human EGF fusion protein enhances the transformation of fibroblasts to fibro sarcomas and induces the development of HCC (Xiao et al., 2017).
A functional polymorphism in EGF at position 61 (A > G) was recently identified with the G/G genotype associating with higher gene expression compared to the A/A genotype Similarly, increased EGF expression was reported in serum and liver tissue from HCC patients with the G/G genotype . This case control study aimed to investigate the association between the risk of hepatocellular carcinoma and the polymorphism of the epidermal growth factor (EGF) gene and detection of EGF expression in HCC tissue and its relation to the polymorphism of this gene. Total of 100 subjects were enrolled in this case-control study. The subjects involved in this study were classified according to clinical examination, laboratory and imaging findings into 3 groups, as follows: (group I): 50 cirrhotic HCV patients with (HCC), subdivided to: IA: 25 patients with surgical rescetable HCC. IB: 25 patients with advanced unrespectable HCC (Multicentric hepatic focal lesions with and/ or portal vein thrombosis). (group II): 25 cirrhotic HCV patients with no evidence of HCC. (group III): 25 healthy persons as control group
All subjects were submitted to the followings:
Full history taking, full clinical examinations for disease diagnosis, Radiological investigation including U/S for tumor size, Triphasic CT, Child-Pugh classification for patient groups and Routine Laboratory Investigations including: CBC, Liver Function tests, HCV antibody and HBs Ag, αFP , Epidermal growth factor polymorphism analysis by PCR- RFLP technique and Immunohistochemically detection of EGF in tissue specimens HCC patients those underwent liver resection after receiving informed consent.