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العنوان
Immunological and molecular evaluation of bacterial human pathogens in drinking waters /
المؤلف
Wajer, Hayder juamia.
هيئة الاعداد
باحث / حيدر جمعة وجر
مشرف / يحي عبد المنعم عثمان اللازق
مشرف / هشام محمد الشيشتاوي
مشرف / هشام محمد الشيشتاوي
الموضوع
Microbiology. Immunological - Drinking waters. Bacterial human - Pathogens.
تاريخ النشر
2019.
عدد الصفحات
online resource (86 pages) :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة المنصورة - كلية العلوم - قسم الميكروبيولوجي
الفهرس
Only 14 pages are availabe for public view

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Abstract

The availability of high quality water is one of the fundamental rights of all human beings. International standards stipulate that drinking water should be tasteless, odorless, colorless and free from pathogenic microbes. In this study, using traditional microbiological techniques, twelve bacterial colonies were isolated and identified from eight samples of drinking water collected from different areas in Dakahlia and Alexandria governorates. Molecular biology techniques such as multi-finger polygonal DNA fingerprints, protein range patterns, and gene encoding sequences, 16S rRNA, and Matrix-Assisted Lasar Desorption Ionization were used to identify bacteria resistant to many drugs and isolated from drinking water collected.It was found that all bacterial isolates belong to three genera, E. coli, Staphylococcus epidermis and Bacillus. Definition of bacteria compared to other molecular biology techniques, if bacterial spectra are available in the library of the Maldives used. Detection of nurse bacteria in drinking waterComparison of classical methods with modern methods used to detect DNA-based pathogenic bacteria for accuracy in bacterial identification. Identify cases of human pathogens bacteria in drinking water.In order to achieve these objectives, eight samples were selected from different regions of Dakahlia and Alexandria After cultivation in the bacteriological laboratory at the Faculty of Science, Mansoura University, 17 isolates of pure bacteria were obtained. After that, some experiments were carried out to determine the shape and size of the bacteria by dyeing Gram. 1.Inhibit transplantation in cruise queues and results have occurred worldwide. Are you unable2. The first test is the protein analysis of these antibiotic-resistant dielectric isolates as previously reported with the two of these samples. Insulators 1, 3, 6, 7, and 11 are closely similar and insulators 2, 4 and 12 are similar.3. All bacterial isolates have been subjected to antibiotic sensitivity test as the presence of these isolates is an indicator of their seriousness to human health. The test was carried out using seven different antibiotic tablets: vancomycin (30 mg), penicillin (10 mg), kanmacin (30 mg), erythromycin (15 mg), trimethoprimol sulva (1.25-23.75mg), genetamis (10mg) and ampicillin (10mg). 4. After that was the modern methods and techniques to identify and identify bacteria, including genetic fingerprint as well as the fingerprint protease as well as the fingerprint pound 16s rRNA.It has been shown from previous studies that drinking water samples can be exposed to environmental pollution during their transition to housing and pipes and this explains their containment of some harmful bacteria present in them can directly affect humans and animals, and therefore continued use of drinking water and continued contamination through the wrong uses Affect human health over the long run. The results showed that the emergence of different types of bacteria between different samples and also the emergence of a difference between them in terms of resistance and sensitivity to antibiotics as well as the accuracy and speed of modern methods of detection and identification and classification of these organisms as well as cost..Key words: Antigram, DNA fingerprint, protein profile , 16s rRNA.