الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Part one:
Formulation, optimization and evaluation of sustained release fisetin loaded polymeric nanoparticles.Fisetin loaded nanoparticles have been prepared using poly (hydroxybutyrate-co-hydroxyvalerate). Optimization of the most important formulation and processing parameters that affect such formulation was assessed. Morphological and spectrophotometric as well as particle size and zeta potential were measured. In vitrorelease study of fisetin from these nanoparticles was conducted. The results revealed that the preparation process was occurred with accepted particle size and there is no chemical interaction between fisetin and the polymer in addition to the sustained release profile compared to free fisetin.
Part two:
Polymeric nanoparticles loaded with combination of fisetin and disulfiram as a promising treatment for colorectal cancer.Poly(hydroxybutyrate-co-hydroxyvalerate) nanoparticles were dually loaded with fisetin and disulfiram then coated with successive layer of chitosan and hyaluronic acid.
Spectrophotometric, morphological, thermal and physicochemical characterizations as well as in vitro release study were investigated. In vitro cytotoxicity against two colorectal cancer cell lines in addition to In vivo pharmacokinetic evaluation of fisetin based nanoformulations were studied. The results showed that chitosan-hyaluronic acid coated dual mono and dual drug loaded nanoparticles have superior characteristic over the free drug(s) in term of cytotoxicity and pharmacokinetic parameters.