الفهرس 
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Abstract
This in vitro study was conducted to evaluate different biological, antimicrobial, physical and mechanical properties of different concentration of GO in experimental GO/PMMA mixture. Nano graphene oxide was incorporated in different concentrations 0%, 0.05%, 0.1%, and 0.15% into commercial available PMMA powder to form groups from I to V respectively.
A total of 640 specimens (160 for each group) were prepared by mixing PMMA powder to MMA monomer. The ratio of Powder: Liquid was 2.5:1 by weight. For evaluation of initial biocompatibility rabbit hemolysis test for blood collected from three healthy adult male New Zealand rabbits (Oryctolagus cuniculus) was conducted. The antimicrobial property was assisted through agar well diffusion test on blood agar media for SM EMCC 1815T (ATCC25175) and Sabouraud dextrose agar for CA ATCC 10231 and isolated strain of CA from denture stomatitis.
Physical properties were assisted throughout evaluation of linear polymerization shrinkage after 1 day and 7 days, effect of water sorption on linear dimensions, α, water sorption and solubility. Mechanical properties were assisted throughout evaluation of tensile strength, compressive strength, flexural strength, roughness, hardness and wear.
The obtained numerical values from all tests were statically analyzed and correlations between data were calculated. According to ANSI/ADA specification no.41 all tested groups were considered as non-hemolytic as their hemolysis percentages were less than 5%. For antimicrobial evaluation all tested groups for all microorganisms showed no inhibition zone except for group II against SM.
For physical properties evaluation with the increasing of the GO concentration from group I to IV the results showed, insignificant reduction in polymerization shrinkage and effect of water sorption on the linear dimensions. Significant reduction in α measurements with increasing the concentration of GO was also showed. Finally insignificant increasing in water sorption and solubility was observed.
For mechanical properties evaluation with the increasing of the GO concentration from group I to IV the results showed, insignificant increasing in tensile strength, compressive strength, flexural strength, and hardness. Insignificant reduction in roughness and significant reduction in wear were also observed.
The correlation between 7 days polymerization shrinkage and effect of water sorption on dimensions showed significant weak positive relationship. The correlation between water sorption and water solubility and between water sorption and effect of water sorption on dimension significant were significant strong positive relationship. The correlation between water solubility and effect of water sorption on dimension significant showed medium negative relationship.
For the mechanical surface related properties the correlation showed significant medium negative relationship between hardness and wear, insignificant weak negative relationship between hardness and roughness and significant weak positive relationship between roughness and wear.