الفهرس 
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Abstract
The problem statement: Foot and mouth disease threatens the animal wealth in Egypt, makes an economic loss, and the reference diagnostic procedures lack sensitivity and selectivity. Besides the performing time and cost of these diagnostic kits.
Aim of this work: providing a rapid, sensitive, and selective diagnostic procedure performed in a very short time on a chip matrix (Lab-on-a-chip).
These goals performed as the following:
The resulted data revealed that:
The proposed FMDV biosensor is a simple, rapid, and more reliable system for FMDV diagnosis due to:
- The MIP film of the POAP as a bio-recognition element for FMDV serotype O (plastic antibodies).
- The proposed biosensor is selective for FMDV serotype O in presence of other viruses.
- The sensitivity and the capturing capacity effective and the resulted limit of detection & quantification (LOD) & (LOQ) were 2 ng/mL and 6 ng/mL, respectively.
- On the application on real samples, the proposed virus biosensor revealed a sensitivity of the virus particles with quantification according to the estimated calibration curve.
- The technique was performed with high sensitivity, accurate and selectivity within 5 minutes which is reliable in emergency cases.
The conclusion:
VIP biosensor that has been developed in the present study to offer very low LOD with 1.98 ng/mL. In comparison to the reference methods such as ELISA and PCR, higher sensitivity with about 50-fold was successfully obtained. The fact of the very fast response taking around 5 min in addition to the re-usability of the biosensor developed in the current study for qualitative and quantitative detection of FMDV, presents a promising, affordable and economic tool that can be integrated with a portable electrochemical potentiostat to be used in field without any need to perform complicated and expensive laboratory experiments.