الفهرس 
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Abstract
The main objective of this study is to find the molecular diversity between the recent isolated Egyptian variant and the vaccine strains used for IBV vaccination in Egypt in attempting to make a good control for limiting the spreading of IBV.
In the existing study, 81 tracheal tissues from 27 vaccinated broiler chicken flocks exhibited respiratory signs and high mortality rate up to 80% were collected from 8 Egyptian governorates during the period from 2017 to 2018.
These samples were collected from eight Egyptian governorates (Sharkia, Qalyubiya, Dakahliya, North Sinai, Suez, Kafrelsheikh, Ismailia, and Giza). The study designed to inspect the Egyptian broiler chicken flocks for detection of IBV infection and study the genetic variations between isolated field strains and their compatibility with different available commercially used vaccine strains.
Out of the twenty-seven examined flocks, 9 flocks were positive for IBV using reverse transcriptase- Real Time polymerase chain reaction (rRT-PCR). The virus isolation from the rRT-PCR positive samples was carried out in ECEs through chorioallantoic fluids up to four blind passages to obtain the pathognomonic feature of IBV infection representing in curling, dwarfing and stunting as well as screening of AF of nine positive samples by Rapid Slid HA test when be tested by 10% washed chicken red blood cells found that it was negative for rapid slide HA activity.