الفهرس 
Isolation, Enhancing, Molecular and Biochemical Identification of Atrazine Metabolizing Bacterial Isolates from SoilOnly 14 pages are availabe for public view
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Abstract
The main goal of thesis was planned to introduce an environmentally friendly technique to solve atrazine contamination problem in different environmental resource. Thus different
bacterial isolates from two types of soil differentially in atrazine applications history were isolated. Two consortiums from each soil sample (Sandy loam and Clay loam) were obtained
using non-selective and selective media. The differentiation between isolates was conducted
based on some characteristics like shape, gram reaction, and spore formation. The 1st soil isolates
were divided to three isolates as gram positive and eight isolates were gram negative. According
to the cell shape seven isolates were presented as rod shape while the other isolates were forming
cocci shape. Moreover, all isolates did not form spores.
Eleven morphologically different phenotypes were selected and purified from second soil.
Six isolates were gram positive, while the other isolates were found to be gram negative.
According to the cell shape six isolates were rod shape while the other isolates were showed a
cocci style. Moreover, all bacterial isolates which isolated from the second soil did not form
spores as well as the other soil.
Isolates efficiency were assayed with two different methods: UV and turbidity. In the first
method the concentration of remaining atrazine in supernatant after incubation with bacterial
isolate was determine while in the second one, bacterial growth was measured at 600 nm as a
result of incubation with atrazine.
The consortium AyDds isolated from the soil of a long history of atrazine applications by selective media was capable of degrading 82.97% of applied atrazine in 60 days. While the consortium AyDt isolated from the soil of a minor history of atrazine applications by nonselective
media showed the lowest atrazine degradation percentage (56.67%) at the same incubation time.