الفهرس | Only 14 pages are availabe for public view |
Abstract This work was carried out to study the bacteriological importance of Cronobacter sakazakii as a potential foodborne emerging pathogen involved in severe illness and deaths in humans, especially neonates. Isolation of Cronobacter spp. in different samples collected from Zagazig city, Sharkia province, Egypt: • A total of 100 samples [PIF milk (n=55), PIF food (n=15), milk powder (n=15) and milk powder products (n=15) were collected and subjected to bacteriological examination for the presence of Cronobacter. • Six samples out of 100 examined (6%) were found positive for Cronobacter spp. • The isolation rates were 5% in PIF milk and 1% in PIF food • The identity of the isolated organism was confirmed as Cronobacter spp. by subjecting the bacteriologically positive samples to PCR technique using 16S rRNA species specific primers. • Cronobacter specific 16S rRNA was detected respectively in 3/5 and 1/5 of bacteriologically positive PIF milk and PIF food examined. • All positive 16S rRNA (n=4) were examined for the presence of C. sakazakii. • C. sakazakii were confirmed in an isolate from 3 isolates of PIF milk and one of PIF food. Molecular characterization and detection of virulence genes (ompA and zpx) in C. sakazakii. • The confirmed Cronobacter (n=4) were examined for harboring the virulent genes ompA and zpx. • ompA gene was detected in 2 identified C. sakazakii isolates. While, 3 Cronobacter isolates were negative for ompA. However, the zpx virulence associated gene was identified in one C. sakazakii isolate. Antibiotic susceptibility of Cronobacter isolates to different antibiotics: • All Cronobacter isolates in the current study were subjected to antibiotic susceptibility test to 8 antibiotics of different groups. • For C. sakazakii, all C. sakazakii isolates were sensitive to ciprocin, while 75% of the isolates were resistant to rifampicin and streptomycin. |